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Collagen-Immobilized Lipases Show Good Activity and Reusability for Butyl Butyrate Synthesis

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Abstract

Candida rugosa lipases were immobilized onto collagen fibers through glutaraldehyde cross-linking method. The immobilization process has been optimized. Under the optimal immobilization conditions, the activity of the collagen-immobilized lipase reached 340 U/g. The activity was recovered of 28.3 % by immobilization. The operational stability of the obtained collagen-immobilized lipase for hydrolysis of olive oil emulsion was determined. The collagen-immobilized lipase showed good tolerance to temperature and pH variations in comparison to free lipase. The collagen-immobilized lipase was also applied as biocatalyst for synthesis of butyl butyrate from butyric acid and 1-butanol in n-hexane. The conversion yield was 94 % at the optimal conditions. Of its initial activity, 64 % was retained after 5 cycles for synthesizing butyl butyrate in n-hexane.

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Acknowledgments

The authors are grateful for the financial support of National High Technology Research and Development Program of China (No. 2013AA06A306).

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Authors and Affiliations

  1. The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu, 610065, China

    Song Dewei, Chen Min & Cheng Haiming

  2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu, 610065, China

    Chen Min & Cheng Haiming

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  1. Song Dewei
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Correspondence to Cheng Haiming.

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Dewei, S., Min, C. & Haiming, C. Collagen-Immobilized Lipases Show Good Activity and Reusability for Butyl Butyrate Synthesis. Appl Biochem Biotechnol 180, 826–840 (2016). https://doi.org/10.1007/s12010-016-2136-2

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