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Regeneration-Based Quantification of Coumarins (Scopoletin and Scoparone) in Abutilon indicum In Vitro Cultures

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Abstract

Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on subsequent introduction of 0.2 mg/l TDZ into the Kn-supplemented media, giving rise to somatic embryos. The embryogenic potential of calli expressed in terms of embryo-forming capacity (EFC) increased from 8.15 EFC to 20.95 EFC after plasmolysis. The phytochemical analysis (HPLC) for the presence of scopoletin and scoparone has revealed a unique accumulation pattern, with higher levels of scopoletin during the earlier stages and scoparone in the later stages of development. The embryogenic calli contained the highest amount of coumarins (99.20 ± 0.97 and 61.03 ± 0.47 μg/gFW, respectively) followed by regenerated plant (9.43 ± 0.20 and 36.36 ± 1.19 μg/gFW, respectively), obtained via somatic embryogenesis. Rapid multiplication of A. indicum equipped with two potent coumarins is important in order to meet the commercial demand for combat against dreadful diseases, thereby providing a new platform for plant-based drugs and their manufacture on a commercial scale.

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Acknowledgments

This work was funded by the University Grants Commission (UGC) in the form of Dr. D.S. Kothari postdoctoral fellowship to Dr. Kiranmayee Rao under the grant number [no.F.4-2/2006(BSR)/13-738/2012(BSR)].

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  1. Department of Genetics, Faculty of Science, Osmania University, Hyderabad, 500 007, India

    Kiranmayee Rao, Bhuvaneswari Chodisetti & P. B. Kavi Kishor

  2. Centre for Biotechnology, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India

    Suryakala Gandi & Archana Giri

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  1. Kiranmayee Rao
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Rao, K., Chodisetti, B., Gandi, S. et al. Regeneration-Based Quantification of Coumarins (Scopoletin and Scoparone) in Abutilon indicum In Vitro Cultures. Appl Biochem Biotechnol 180, 766–779 (2016). https://doi.org/10.1007/s12010-016-2131-7

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