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Improving Erythritol Production of Aureobasidium pullulans from Xylose by Mutagenesis and Medium Optimization

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Abstract

The biotransformation of xylose, the second most abundant sugar, has been a hot topic in recent years. In this work, Aureobasidium pullulans CGMCC3.0837 was subjected to UV mutagenesis to improve its erythritol production from xylose. The erythritol production of the obtained mutant ER35 was 50.92 % (17.28 g/L) higher than that of the parent strain. Response surface methodology was applied to optimize the medium composition. Yeast extract, KH2PO4, and citric acid were the key factors influencing erythritol synthesis, and the optimal concentrations were 17.82, 0.76, and 6.60 g/L, respectively. Under the optimized conditions, 31.75 and 31.42 g/L erythritol were obtained in shake flasks and in a 5-L fermentor, respectively. ER35 also showed a good consuming ability on xylose mother liquor with a final erythritol production of 26.35 g/L. This report provided insights into the potential of A. pullulans for the production of erythritol using xylose as a carbon source.

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Acknowledgments

The research was supported by the “Twelfth Five-Year” National Science and technology project in rural areas (grant no. 2012AA101805).

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Jian Guo, Junxia Li, Yefu Chen, Xuewu Guo & Dongguang Xiao

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  1. Jian Guo
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  2. Junxia Li
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  3. Yefu Chen
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  4. Xuewu Guo
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  5. Dongguang Xiao
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Correspondence to Yefu Chen.

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Guo, J., Li, J., Chen, Y. et al. Improving Erythritol Production of Aureobasidium pullulans from Xylose by Mutagenesis and Medium Optimization. Appl Biochem Biotechnol 180, 717–727 (2016). https://doi.org/10.1007/s12010-016-2127-3

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  • DOI: https://doi.org/10.1007/s12010-016-2127-3

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