Microbial Degradation of Chlorogenic Acid by a Sphingomonas sp. Strain
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In order to elucidate the metabolism of chlorogenic acid by environmental microbes, a strain of Sphingomonas sp. isolated from tobacco leaves was cultured under various conditions, and chlorogenic acid degradation and its metabolites were investigated. The strain converting chlorogenic acid was newly isolated and identified as a Sphingomonas sp. strain by 16S rRNA sequencing. The optimal conditions for growth and chlorogenic acid degradation were 37 °C and pH 7.0 with supplementation of 1.5 g/l (NH4)2SO4 as the nitrogen source and 2 g/l chlorogenic acid as the sole carbon source. The maximum chlorogenic acid tolerating capability for the strain was 5 g/l. The main metabolites were identified as caffeic acid, shikimic acid, and 3,4-dihydroxybenzoic acid based on gas chromatography-mass spectrometry analysis. The analysis reveals the biotransformation mechanism of chlorogenic acid in microbial cells isolated from the environment.
KeywordsDegradation Chlorogenic acid Intermediate metabolites Sphingomonas sp.
This work was supported in part by grants from the Chinese National Science Foundation for Excellent Young Scholars (31422004), the Chinese National Natural Science Foundation (31270154).
- 5.Pietraforte, D., Castelli, M., Metere, A., Scorza, G., Samoggia, P., Menditto, A., & Minetti, M. (2006). Salivary uric acid at the acidic pH of the stomach is the principal defense against nitrite-derived reactive species: sparing effects of chlorogenic acid and serum albumin. Free Radical Biology and Medicine, 41, 1753–1763.CrossRefGoogle Scholar
- 7.Huang, M.-T., Smart, R. C., Wong, C.-Q., & Conney, A. H. (1988). Inhibitory effect of curcumin, chlorogenic acid, caffeic acid, and ferulic acid on tumor promotion in mouse skin by 12-O-tetradecanoylphorbol-13-acetate. Cancer Research, 48, 5941–5946.Google Scholar
- 10.Chlopčíková, Š., Psotová, J., Miketová, P., Soušek, J., Lichnovský, V., & Šimánek, V. (2004). Chemoprotective effect of plant phenolics against anthracycline-induced toxicity on rat cardiomyocytes part II. caffeic, chlorogenic and rosmarinic acids. Phytotherapy Research, 18, 408–413.CrossRefGoogle Scholar
- 13.Olthof, M. R., Hollman, P. C. H., Buijsman, M. N. C. P., Van Amelsvoort, J. M. M., & Katan, M. B. (2003). Chlorogenic acid, quercetin-3-rutinoside and black tea phenols are extensively metabolized in humans. The Journal of Nutrition, 133, 1806–1814.Google Scholar
- 17.Peppercorn, M. A., & Goldman, P. (1971). Caffeic acid metabolism by bacteria of the human gastrointestinal tract. Journal of Bacteriology, 108, 996–1000.Google Scholar
- 24.Karunanidhi, A., Thomas, R., Van Belkum, A., & Neela, V. (2013). In vitro antibacterial and antibiofilm activities of chlorogenic acid against clinical isolates of Stenotrophomonas maltophilia including the trimethoprim/sulfamethoxazole resistant strain. BioMed Research International, 2013, 7.CrossRefGoogle Scholar
- 25.Gauthier, L., Bonnin-Verdal, M.-N., Marchegay, G., Pinson-Gadais, L., Ducos, C., Richard-Forget, F., & Atanasova-Penichon, V. (2016). Fungal biotransformation of chlorogenic and caffeic acids by Fusarium graminearum: new insights in the contribution of phenolic acids to resistance to deoxynivalenol accumulation in cereals. International Journal of Food Microbiology, 221, 61–68.CrossRefGoogle Scholar