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The Addition of N-Hexanoyl-Homoserine Lactone to Improve the Microbial Flocculant Production of Agrobacterium tumefaciens Strain F2, an Exopolysaccharide Bioflocculant-Producing Bacterium

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Abstract

In this study, N-hexanoyl-homoserine lactone (C6-HSL), a member of the N-acyl-homoserine lactone class of microbial quorum sensing (QS) signaling molecules, was used to improve microbial flocculant production. After exogenous C6-HSL was added, exopolysaccharide concentration of microbial flocculants was improved by 1.6-fold and flocculation rate of microbial flocculants was increased by 10 %. Fermentation conditions with added C6-HSL were further optimized through response surface methodology. The obtained optimal fermentation conditions were as follows: added C6-HSL concentration of 0.45 μM, fermentation temperature of 30.4 °C, and initial fermentation pH of 7.25. Under these optimal fermentation conditions, the resulting exopolysaccharide concentration was improved by 1.75-fold and flocculation rate was increased by 10 % compared with that of the control group. The yield of microbial flocculants was also improved by 1.75-fold. Results demonstrated that the existence of QS system in Agrobacterium tumefaciens strain F2 played the important roles in the microbial flocculant production.

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Acknowledgments

This work was supported by grants from the Fundamental Research Funds for the Central Universities (No. HIT. NSRIF. 2015095), the National Natural Science Foundation of China (No. 51578179), and the Postdoctoral Scientific Research Development Fund of Heilongjiang Province in 2014 (No. LBH-Q14076).

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Authors and Affiliations

  1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, People’s Republic of China

    Jixian Yang, Dan Wu, Ang Li, Haijuan Guo, Han Chen, Shanshan Pi, Wei Wei & Fang Ma

  2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People’s Republic of China

    Jixian Yang, Dan Wu, Ang Li, Han Chen, Shanshan Pi, Wei Wei & Fang Ma

  3. School of Civil Engineering and Architecture, Taizhou University, Taizhou, 318000, People’s Republic of China

    Haijuan Guo

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  1. Jixian Yang
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  2. Dan Wu
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  3. Ang Li
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  4. Haijuan Guo
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  5. Han Chen
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  7. Wei Wei
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  8. Fang Ma
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Correspondence to Ang Li or Haijuan Guo.

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Yang, J., Wu, D., Li, A. et al. The Addition of N-Hexanoyl-Homoserine Lactone to Improve the Microbial Flocculant Production of Agrobacterium tumefaciens Strain F2, an Exopolysaccharide Bioflocculant-Producing Bacterium. Appl Biochem Biotechnol 179, 728–739 (2016). https://doi.org/10.1007/s12010-016-2027-6

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  • DOI: https://doi.org/10.1007/s12010-016-2027-6

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