Abstract
In this study, N-hexanoyl-homoserine lactone (C6-HSL), a member of the N-acyl-homoserine lactone class of microbial quorum sensing (QS) signaling molecules, was used to improve microbial flocculant production. After exogenous C6-HSL was added, exopolysaccharide concentration of microbial flocculants was improved by 1.6-fold and flocculation rate of microbial flocculants was increased by 10 %. Fermentation conditions with added C6-HSL were further optimized through response surface methodology. The obtained optimal fermentation conditions were as follows: added C6-HSL concentration of 0.45 μM, fermentation temperature of 30.4 °C, and initial fermentation pH of 7.25. Under these optimal fermentation conditions, the resulting exopolysaccharide concentration was improved by 1.75-fold and flocculation rate was increased by 10 % compared with that of the control group. The yield of microbial flocculants was also improved by 1.75-fold. Results demonstrated that the existence of QS system in Agrobacterium tumefaciens strain F2 played the important roles in the microbial flocculant production.
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This work was supported by grants from the Fundamental Research Funds for the Central Universities (No. HIT. NSRIF. 2015095), the National Natural Science Foundation of China (No. 51578179), and the Postdoctoral Scientific Research Development Fund of Heilongjiang Province in 2014 (No. LBH-Q14076).
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Yang, J., Wu, D., Li, A. et al. The Addition of N-Hexanoyl-Homoserine Lactone to Improve the Microbial Flocculant Production of Agrobacterium tumefaciens Strain F2, an Exopolysaccharide Bioflocculant-Producing Bacterium. Appl Biochem Biotechnol 179, 728–739 (2016). https://doi.org/10.1007/s12010-016-2027-6
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DOI: https://doi.org/10.1007/s12010-016-2027-6