Abstract
The present study describes the improved mycelia and exo-polymer production under control of Grifola frondosa morphology by changing the aeration rate and agitation intensity in a 25-L stirred fermentor. The aeration rate of 1.0 vvm yielded a highest mycelia biomass of 24.754 g/L with the lowest pellet percentage of 20.5 %. The maximum exo-polymer (2.324 g/L) was achieved at 0.75 vvm with mycelia polysaccharide production (0.321 g/g), whereas clumps and filaments dominated the ratios of 45.6 and 33.9 %, respectively. The change of aeration rate and agitation intensity had slight influence on the monosaccharide compositions in exo-polymers and significantly affected glucose and mannose contents in the mycelia polysaccharides. These findings will provide a clue for exploring the relationship between fermentation parameters, morphologies, and polysaccharide synthesis pathway of G. frondosa.
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Acknowledgments
This work was supported by funding from the Natural Science Foundation of China (NSFC 31101269), China Postdoctoral Science Special Foundation (2013T60648), 2012 Excellent Key Young Teachers Project of Jiangsu University, Science & Technology Platform Construction Program of Jiangxi Province, and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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Fig. S1
Typical morphological changes of G. frondosa GF9801 in a 25-L stirred fermentor at different agitation intensities of 60 rpm (a), 90 rpm (b), 120 rpm (c), and 150 rpm (d) during the 7-day fermentation period at 0.5 vvm and 28 °C. The bar is 1 mm. (DOCX 11,748 kb)
Fig. S2
Typical morphological changes of G. frondosa GF9801 in a 25-L stirred fermentor at different aeration rates of 0.25 vvm (a), 0.5 vvm (b), 0.75 vvm (c), and 1.0 vvm (d) during the 7-day fermentation period at 90 rpm and 28 °C. Bar = 1 mm. (DOCX 6816 kb)
Table S1
Morphological parameters of pellets/mycelial aggregates of G. frondosa at different agitation intensities (DOCX 35 kb)
Table S2
Morphological parameters of pellets/mycelial aggregates of G. frondosa at different aeration rates. (DOCX 35 kb)
Table S3
Effects of aeration rates and agitation intensities on monosaccharide composition of mycelia polysaccharide produced by G. frondosa GF 9801. (DOCX 36 kb)
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Cui, FJ., Chen, XX., Liu, WM. et al. Control of Grifola frondosa Morphology by Agitation and Aeration for Improving Mycelia and Exo-Polymer Production. Appl Biochem Biotechnol 179, 459–473 (2016). https://doi.org/10.1007/s12010-016-2006-y
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DOI: https://doi.org/10.1007/s12010-016-2006-y