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Applied Biochemistry and Biotechnology

, Volume 179, Issue 1, pp 94–110 | Cite as

Synthesis and Characterisation of Biocompatible Polymer-Conjugated Magnetic Beads for Enhancement Stability of Urease

  • Yasemin Ispirli DoğaçEmail author
  • Mustafa Teke
Article

Abstract

We reported natural polymer-conjugated magnetic featured urease systems for removal of urea effectively. The optimum temperature (20–60 °C), optimum pH (3.0–10.0), kinetic parameters, thermal stability (4–70 °C), pH stability (4.0–9.0), operational stability (0–250 min), reusability (18 times) and storage stability (24 weeks) were studied for characterisation of the urease-encapsulated biocompatible polymer-conjugated magnetic beads. Also, the surface groups and chemical structure of the magnetic beads were determined by using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The all urease-encapsulated magnetic beads protected their stability of 30–45 % relative activity at 70 °C. A significant increase was observed at their pH stability compared with the free urease for both acidic and alkaline medium. Besides this, their repeatability activity were approximately 100 % during 4th run. They showed residual activity of 50 % after 16 weeks. The importance of this work is enhancement stability of immobilised urease by biocompatible polymer-conjugated magnetic beads for the industrial application based on removal of urea.

Keywords

Fe3O4 nanoparticles Fe[NiFe]O4 nanoparticles Magnetic polymeric beads Urease encapsulation Urease stability 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Faculty of Science, Chemistry DepartmentMuğla Sıtkı Koçman UniversityMuğlaTurkey

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