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Exploring the Effects of Subfreezing Temperature and Salt Concentration on Ice Growth Inhibition of Antarctic Gram-Negative Bacterium Marinomonas Primoryensis Using Coarse-Grained Simulation

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Abstract

The aim of this work is to study the freezing process of water molecules surrounding Antarctic Gram-negative bacterium Marinomonas primoryensis antifreeze protein (MpAFP) and the MpAFP interactions to the surface of ice crystals under various marine environments (at different NaCl concentrations of 0.3, 0.6, and 0.8 mol/l). Our result indicates that activating temperature region of MpAFPs reduced as NaCl concentration increased. Specifically, MpAFP was activated and functioned at 0.6 mol/l with temperatures equal or larger 278 K, and at 0.8 mol/l with temperatures equal or larger 270 K. Additionally, MpAFP was inhibited by ice crystal network from 268 to 274 K and solid–liquid hybrid from 276 to 282 K at 0.3 mol/l concentration. Our results shed lights on structural dynamics of MpAFP among different marine environments.

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Acknowledgments

The work was funded by the Department of the Navy, Office of Naval Research under grant number N62909-14-1-N234. The computing resources and support provided by Institute for Computational Science and Technology, Ho Chi Minh City, Vietnam are gratefully acknowledged.

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Authors and Affiliations

  1. Life Science Laboratory of Institute for Computational Science and Technology, Ho Chi Minh City, Vietnam

    Hung Nguyen, Thanh Dac Van, Nhut Tran & Ly Le

  2. School of Biotechnology of Ho Chi Minh International University, Vietnam National University, Ho Chi Minh City, Vietnam

    Thanh Dac Van & Ly Le

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  1. Hung Nguyen
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  2. Thanh Dac Van
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Correspondence to Hung Nguyen or Ly Le.

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Nguyen, H., Dac Van, T., Tran, N. et al. Exploring the Effects of Subfreezing Temperature and Salt Concentration on Ice Growth Inhibition of Antarctic Gram-Negative Bacterium Marinomonas Primoryensis Using Coarse-Grained Simulation. Appl Biochem Biotechnol 178, 1534–1545 (2016). https://doi.org/10.1007/s12010-015-1966-7

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  • DOI: https://doi.org/10.1007/s12010-015-1966-7

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