Abstract
Discovery of novel secreted enzymes and proteins in Mycobacterium tuberculosis (M. tuberculosis) are imperative to understanding the pathogenic system for pathogenesis requires attention. Till date, the groups of these secreted enzymes are not meaningfully characterized in terms of M. tuberculosis. In this way, cutinase, a small lipolytic protein, exists in both bacteria and fungi as well which have a potential catalytic activity. During our search, we have found a few genes of M. tuberculosis demonstrating a same significant lipase action as fungi Fusarium solani cutinase contain. Genome sequencing of M. tuberculosis uncover a lot of proteins, wherein (Rv1758, Rv1984c, Rv2301, Rv3451, Rv3452, Rv3724A, Rv3724B, and Rv3802c) genes have been noted which are exhibiting a cutinase-like activity and closely homologous to that of F. solani cutinase and having the ability to hydrolyze model substrates including p-nitrophenyl butyrate (p-PNB), cutin, triacylglycerols (TAGs), and triolein (TO), yet their biological significance in pathogenesis stays subtle and uncharacterized. In a basic perspective, the measure of cutinase expressed by M. tuberculosis and part of these small lipolytic enzymes in the pathologic discipline require thorough characterization. So, through focusing on cutinase-encoding genes in M. tuberculosis and their active catalytic motif could help to build up a novel therapeutic approach.
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Abbreviations
- M. tuberculosis :
-
Mycobacterium tuberculosis
- F. solani :
-
Fusarium solani
- T. fusca :
-
Thermomonospora fusca
- P. spp.:
-
Pseudomonas spp.
- Culp:
-
Cutinase-like proteins
- Cut:
-
Cutinase
- TO:
-
Triolein
- LDs:
-
Lipid droplets
- TAGs:
-
Triacylglycerols
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Acknowledgments
We thank Dr. Rajesh S. Gokhale for making this work possible. The authors acknowledge the financial support from GAP0092 of the Department of Science and Technology (DST) and OLP1121of the Council of Scientific & Industrial Research (CSIR).
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Monu, Meena, L.S. Roles of Triolein and Lipolytic Protein in the Pathogenesis and Survival of Mycobacterium tuberculosis: a Novel Therapeutic Approach. Appl Biochem Biotechnol 178, 1377–1389 (2016). https://doi.org/10.1007/s12010-015-1953-z
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DOI: https://doi.org/10.1007/s12010-015-1953-z