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Enzymatic Hydrolysis of Hydrotropic Pulps at Different Substrate Loadings

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Abstract

Enzymatic hydrolysis of cellulosic raw materials to produce nutrient broths for microbiological synthesis of ethanol and other valuable products is an important field of modern biotechnology. Biotechnological processing implies the selection of an effective pretreatment technique for raw materials. In this study, the hydrotropic treatment increased the reactivity of the obtained substrates toward enzymatic hydrolysis by 7.1 times for Miscanthus and by 7.3 times for oat hulls. The hydrotropic pulp from oat hulls was more reactive toward enzymatic hydrolysis compared to that from Miscanthus, despite that the substrates had similar compositions. As the initial substrate loadings were raised during enzymatic hydrolysis of the hydrotropic Miscanthus and oat hull pulps, the concentration of reducing sugars increased by 34 g/dm3 and the yield of reducing sugars decreased by 31 %. The findings allow us to predict the efficiency of enzymatic hydrolysis of hydrotropic pulps from Miscanthus and oat hulls when scaling up the process by volume.

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Authors and Affiliations

  1. Laboratory of Bioconversion, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 1 Socialisticheskaya St, Biysk, 659322, Altai Krai, Russia

    Marina N. Denisova, Ekaterina I. Makarova, Igor N. Pavlov, Vera V. Budaeva & Gennady V. Sakovich

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  1. Marina N. Denisova
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  2. Ekaterina I. Makarova
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  3. Igor N. Pavlov
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  4. Vera V. Budaeva
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  5. Gennady V. Sakovich
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Correspondence to Marina N. Denisova.

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Denisova, M.N., Makarova, E.I., Pavlov, I.N. et al. Enzymatic Hydrolysis of Hydrotropic Pulps at Different Substrate Loadings. Appl Biochem Biotechnol 178, 1196–1206 (2016). https://doi.org/10.1007/s12010-015-1938-y

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  • DOI: https://doi.org/10.1007/s12010-015-1938-y

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