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Three-Dimensional Simulation of Ultrasound-Induced Microalgal Cell Disruption

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Abstract

The three-dimensional distribution (x, y, and z) of ultrasound-induced microalgal cell disruption in a sonochemical reactor was predicted by solving the Helmholtz equation using a three-dimensional acoustic module in the COMSOL Multiphysics software. The simulated local ultrasound pressure at any given location (x, y, and z) was found to correlate with cell disruption of a freshwater alga, Scenedesmus dimorphus, represented by the change of algal cell particle/debris concentration, chlorophyll-a fluorescence density (CAFD), and Nile red stained lipid fluorescence density (LFD), which was also validated by the model reaction of potassium iodide oxidation (the Weissler reaction). Furthermore, the effect of ultrasound power intensity and processing duration on algal cell disruption was examined to address the limitation of the model.

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Acknowledgments

This research was financially supported by the US National Science Foundation (Award No. CMMI-1239078) and the startup fund of North Carolina State University.

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Authors and Affiliations

  1. Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, NC, 27695, USA

    M. Wang, W. Yuan & Andy Hale

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  1. M. Wang
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  2. W. Yuan
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  3. Andy Hale
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Correspondence to W. Yuan.

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Wang, M., Yuan, W. & Hale, A. Three-Dimensional Simulation of Ultrasound-Induced Microalgal Cell Disruption. Appl Biochem Biotechnol 178, 1184–1195 (2016). https://doi.org/10.1007/s12010-015-1937-z

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  • DOI: https://doi.org/10.1007/s12010-015-1937-z

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