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SSR Markers Associated with Proline in Drought Tolerant Wheat Germplasm

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Abstract

Water stress causes major agricultural loss throughout the world as survival of the crops remained under stress and loss in yield. Plants respond to drought stress by means of different adaptive mechanisms such as accumulation of osmoprotectants to counteract the water stress. Amino acid proline is known to occur widely in higher plants and normally accumulates in large quantities as an osmolyte in response to environmental stresses. Biochemical estimation of proline was done in the drought-affected wheat genotypes by spectrophotometric method. Proline promoted a positive effect as root/shoot ratio was enhanced in wheat germplasm under drought stress. SSR primer pairs (45) were tested for polymorphism among selected wheat genotypes. The dendrogram results have shown the wheat genotype association with the levels of proline during induced drought stress. The relationship between pattern of drought responsive biochemical attributes and DNA markers in the selected wheat genotypes was recognized to select drought tolerant genotypes for sowing in drought affected areas of the country.

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References

  1. Asif, M., Rahman, M., & Zafar, Y. (2005). DNA fingerprinting studies of some wheat (Triticum aestivum L.) genotypes using RAPD analysis. Pakistan Journal of Botany, 37(2), 271–277.

    Google Scholar 

  2. Food and Agriculture Organization 2012. Annual Wheat Report. Internet source: www.fao.org/worldfoodsituation

  3. Zhu, Y., Chen, H., Fan, J., Wang, Y., Li, Y., Chen, J., Fan, J., Yang, S., Hu, L., Leung, H., Mew, T. W., Teng, P. S., Wang, Z., & Mundt, C. C. (2000). Nature, 406, 718–722.

    Article  CAS  Google Scholar 

  4. Nayyar, H. (2004). Variation in osmoregulation in differentially drought sensitive wheat genotype involves calcium. Journal of Biologia Plantarum, 47, 541–547.

    Article  Google Scholar 

  5. Stewart, G. R., & Lee, J. A. (1974). Proline accumulation: biochemical aspects. In “Physiology and Biochemistry of Drought Resistance in Plants”. Planta, 120, 279–289.

    Article  CAS  Google Scholar 

  6. Briens, M., & Larher, F. (1982). Osmoregulation in halophytic higher plants: a comparative study of soluble carbohydrates, polyols, betaines and free proline. Plant, Cell and Environment, 5, 287–292.

    CAS  Google Scholar 

  7. Smirnoff, N., & Cumbes, Q. J. (1989). Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry, 28, 1057–1060.

    Article  CAS  Google Scholar 

  8. Bartels, D. (2002). Drought and desiccation induced modulation of gene expression in plants. Journal of Plant Cell and Environment, 25, 141–151.

    Article  Google Scholar 

  9. Cushman, J. C. (2001). Osmoregulation in plants: implications for agriculture. American Zoologist, 41, 758–769.

    CAS  Google Scholar 

  10. Steudle, E. (2000). Advances in the studies on water uptake by plant roots. Journal of Experimental Botany, 51(530), 1531–1542.

    Article  CAS  Google Scholar 

  11. Iqbal, M. J., Rahman, M., Ashraf, M., Sheikh, M. A., & Jamil, A. (2012). Trehalose expression in hexaploid wheat (Triticum aestivum L.) germplasm under drought stress. Pakistan Journal of life Social Sciences, 10(2), 106–110.

    Google Scholar 

  12. Aziz, A., & Khan, M. A. (2003). Proline and water status of some desert shrubs before and after rains. Pakistan Journal of Botany, 35, 902–906.

    Google Scholar 

  13. Hare, P. D., Cress, W. A., & Staden, J. V. (1996). Dissecting the roles of osmolyte accumulation during stress. Plant, Cell and Environment, 21, 535–553.

    Article  Google Scholar 

  14. Boyer, J. S. (1982). Plant productivity and environment. Science, 218(4571), 443–448.

    Article  CAS  Google Scholar 

  15. Abraham, E., Cabassa, C. H., Erdei, L., & Szabados, L. (2010). Methods for determination of proline in plants. Methods in Molecular Biology, 639, 317–331.

    Article  CAS  Google Scholar 

  16. Hoagland, D.R., & Arnon. D.I. (1950). The water culture method for growing plant without soil. CA. USA, pp. 347.

  17. Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19, 11–15.

    Google Scholar 

  18. Sambrook, J., & Russell, D. W. (2001). Molecular cloning, a laboratory manual (3rd ed.). New York: Cold Spring Harbor.

    Google Scholar 

  19. Stewart, C.R. (1981). Proline accumulation: biochemical aspects. In “Physiology and Biochemistry of Drought Resistance in Plants,” Academic Press, Sydney, pp 243–259.

  20. Nei, M., & Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America, 76, 5269–5273.

  21. Chakravarthy, B. K., & Naravaneni, R. (2006). SSR marker based DNA fingerprinting and diversity study in rice (Oryza sativa. L). African Journal of Biotechnology, 8, 684–688.

    Google Scholar 

  22. Panaud, O., Chen, X., & McCouch, S. R. (1996). Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oriza stiva L.). Molecular and General Genetics, 252, 597–607.

    CAS  Google Scholar 

  23. Ram, S. G., Thiruvengadam, V., & Vinod, K. K. (2007). Genetic diversity among cultivars, landraces and wild relatives of rice as revealed by microsatellite markers. Journal of Applied Genetics, 48, 337–345.

    Article  Google Scholar 

  24. Palombi, M. A., & Damiano, C. (2002). Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Reports, 20, 1061–1066.

    Article  CAS  Google Scholar 

  25. Steel, R. G. D., & Torrie, J. H. (1990). Principles and procedures of statistics. New York. USA: McGraw.

    Google Scholar 

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Correspondence to Amer Jamil.

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Iqbal, M.J., Maqsood, Y., Abdin, Z.U. et al. SSR Markers Associated with Proline in Drought Tolerant Wheat Germplasm. Appl Biochem Biotechnol 178, 1042–1052 (2016). https://doi.org/10.1007/s12010-015-1927-1

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  • DOI: https://doi.org/10.1007/s12010-015-1927-1

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