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A Novel, Poly(Ethyl Ethylene Ether) Inhibitor to Trypsin from Marine Cyanobacteria, Lyngbya confervoides

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Abstract

A novel, poly(ethyl ethylene ether) inhibitor to trypsin was purified from marine cyanobacteria, Lyngbya confervoides from the coastal areas of Thalassery, North Kerala. The kinetics and the thermodynamic parameters of its interactions with the enzyme were also studied. It was demonstrated that the substrate binding, catalytic triad of the enzyme could be blocked by the inhibitor, as expressed by molecular simulation studies. The study also showed that the cyanobacterial group could prove to be a potential source of novel enzyme inhibitors for various applications.

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Acknowledgment

Funding was provided by the Ministry of Earth Science, Government of India at the Department of Biotechnology and Microbiology, Kannur University. The authors acknowledge Institute for Sophisticated Analytical Instrument Facility, KOCHI for NMR facility, and Inter University Center for Instrumentation for LC-MS at Mahatma Gandhi University; Kottayam are acknowledged for data.

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Authors and Affiliations

  1. Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad, 670661, India

    Ambika Devi, Shankar Prasanth, Abdulhameed Sabu & Madhathilkovilakathu Haridas

  2. Centre for Bioinformatics, Department of Bioinformatics, Bharathiar University, Coimbatore, 641046, India

    Easwaran Murugesh

  3. School of Chemical Sciences, Kannur University, Payannur Campus, Edat, 670327, India

    Karickal R. Haridas

Authors
  1. Ambika Devi
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  2. Shankar Prasanth
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  3. Easwaran Murugesh
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  4. Karickal R. Haridas
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  5. Abdulhameed Sabu
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  6. Madhathilkovilakathu Haridas
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Corresponding author

Correspondence to Madhathilkovilakathu Haridas.

Additional information

Research Highlights

• A novel poly(ethyl ethylene ether) was isolated from the marine cyanobacteria, Lyngbya confervoides

• The structure of the compound was deduced by various techniques like IR, LC MS, and NMR spectroscopies.

• Mass of the compound was found to be 948.

• The compound had a profound trypsin inhibition whose interaction was studied by isothermal titration calorimetry, molecular docking, and MD simulations.

• The ligand adopted a Kunitz-type, active-site-directed binding conformation for inhibiting the enzyme trypsin.

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Devi, A., Prasanth, S., Murugesh, E. et al. A Novel, Poly(Ethyl Ethylene Ether) Inhibitor to Trypsin from Marine Cyanobacteria, Lyngbya confervoides . Appl Biochem Biotechnol 178, 891–899 (2016). https://doi.org/10.1007/s12010-015-1916-4

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  • DOI: https://doi.org/10.1007/s12010-015-1916-4

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