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Potential Application of Biohydrogen Production Liquid Waste as Phosphate Solubilizing Agent—A Study Using Soybean Plants

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Abstract

With CO2 free emission and a gravimetric energy density higher than gasoline, diesel, biodiesel, and bioethanol, biohydrogen is a promising green renewable energy carrier. During fermentative hydrogen production, 60–70 % of the feedstock is converted to different by-products, dominated by organic acids. In the present investigation, a simple approach for value addition of hydrogen production liquid waste (HPLW) containing these compounds has been demonstrated. In soil, organic acids produced by phosphate solubilizing bacteria chelate the cations of insoluble inorganic phosphates (e.g., Ca3 (PO4)2) and make the phosphorus available to the plants. Organic acid-rich HPLW, therefore, has been evaluated as soil phosphate solubilizer. Application of HPLW as soil phosphate solubilizer was found to improve the phosphorus uptake of soybean plants by 2.18- to 2.74-folds. Additionally, 33–100 % increase in seed germination rate was also observed. Therefore, HPLW has the potential to be an alternative for phosphate solubilizing biofertilizers available in the market. Moreover, the strategy can be useful for phytoremediation of phosphorus-rich soil.

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Acknowledgments

Centre de Recherche Industrielle du Québec (CRIQ), and Institut National de la Recherche Scientifique (INRS) - Centre Eau, Terre & Environnement (ETE) Canada have been acknowledged for financial support. The authors are also thankful to “merit scholarship program for foreign students (Fonds Québécois de la Recherche sur la Nature et les Technologies)” for financial assistance to S. J. Sarma.

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Authors and Affiliations

  1. Institut National de la Recherche Scientifique (INRS), Centre Eau, Terre & Environnement (ETE), 490, Rue de la Couronne, Québec, QC, G1K 9A9, Canada

    Saurabh Jyoti Sarma & Satinder Kaur Brar

  2. Centre de Recherche Industrielle du Québec (CRIQ), Québec, QC, G1P 4C7, Canada

    Yann LeBihan & Gerardo Buelna

Authors
  1. Saurabh Jyoti Sarma
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  2. Satinder Kaur Brar
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  3. Yann LeBihan
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  4. Gerardo Buelna
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Correspondence to Satinder Kaur Brar.

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Sarma, S.J., Brar, S.K., LeBihan, Y. et al. Potential Application of Biohydrogen Production Liquid Waste as Phosphate Solubilizing Agent—A Study Using Soybean Plants. Appl Biochem Biotechnol 178, 865–875 (2016). https://doi.org/10.1007/s12010-015-1914-6

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  • DOI: https://doi.org/10.1007/s12010-015-1914-6

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