Abstract
Using pharmacological and biochemical approaches, Ca2+ involved in the betulin production in mycelia of Phellinus linteus induced by hydrogen sulfide (H2S) were investigated. The results showed that 2 mM H2S donor NaHS or 10 mM CaCl2 was found to enhance the betulin content in the mycelia of Phellinus to the maximum, which were 112.43 and 93.24 % higher than that in the control, respectively. Further, NaHS and CaCl2 co-treatment also showed positive outcome, which were 128.95 or 24.52 % higher than that in the control or NaHS treatment. At the same time, NaHS also enhanced the content of Ca2+ and CaM. But, the above positive inductive effects for Ca2+, CaM, and betulin production can be blocked with either Ca2+ channel blocker (LaCl3, 2-aminoethoxydiphenyl borate) or Ca2+ chelator (ethylenediaminetetraacetic acid (EDTA)). Among of them, betulin content was reduced 35.06 % by NaHS and EGTA to the minimum, and this reduction could be reversed by the application of CaCl2 (NaHS + EGTA + CaCl2). From above results, it can be concluded that endogenous and exogenous calcium involved in the betulin production from submerged culture of P. linteus induced by hydrogen sulfide.
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Acknowledgments
This work was supported by the Fundamental Research Funds for the Central Universities (2572015EA01), the National Natural Science Foundation of China (31100445), and Harbin Technological Innovation Special Fund research projects (2014RFQXJ066).
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Duan Jian and Meiling Sun contributed equally to this work.
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Supplement Table 1
Differential expression genes of terpenoid backbone biosynthesis in Phellinus linteus responsed to H2S treatment (DOC 38 kb)
Supplement Table 2
Differential expression of Ca2+ related genes in Phellinus linteus responded to H2S treatment (DOC 32 kb)
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Fan, G., Jian, D., Sun, M. et al. Endogenous and Exogenous Calcium Involved in the Betulin Production from Submerged Culture of Phellinus linteus Induced by Hydrogen Sulfide. Appl Biochem Biotechnol 178, 594–603 (2016). https://doi.org/10.1007/s12010-015-1896-4
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DOI: https://doi.org/10.1007/s12010-015-1896-4