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The Effect of Light Stress and Other Culture Conditions on Photoinhibition and Growth of Dunaliella tertiolecta

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Abstract

This work aimed to examine the effects of high light stress as well as other culture conditions including HCO3 − concentration, temperature, salinity, and pre-acclimation on photoinhibition and growth of halotolerant alga Dunaliella tertiolecta. Significant photoinhibition of D. tertiolecta was observed during a short period of exposure (6 hours) to high intensity of lights (1000, 1500, and 2000 μmol photons m−2 s−1); however, after 2 days of continuous light exposure, the alga adapted to high light stress and reached similar growth rates as low light exposure. The increase in HCO3 − concentration in the culture medium did not reduce photoinhibition, but the growth rate and chlorophyll contents increased with increasing HCO3 − concentrations. Temperature had significant effects on photoinhibition. Combined high temperature and high light intensity led to more serious photoinhibition and reduced cell growth rates, so did combined low salinity and high light intensity. Pre-acclimation by 50, 200, or 500 μmol photons m−2 s−1 each for 1, 3, or 6 hours (a total of nine treatments) did not significantly influence photoinhibition or cell growth of D. tertiolecta, probably because the acclimation periods were not long enough.

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Acknowledgments

This research was supported by PTT Research and Technology Institute; the Royal Golden Jubilee Ph.D. Program of Thailand (Thailand Research Fund); the Center for Environmental Health, Toxicology, and Management of Chemicals under the Science & Technology Postgraduate Education and Research Development Office (PERDO) of the Ministry of Education, Thailand; the US National Science Foundation (award # CMMI-1239078); and the startup fund of North Carolina State University.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Jitpisut Seepratoomrosh, Prayad Pokethitiyook, Metha Meetam & Wanvisa Pugkaew

  2. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Kittisak Yokthongwattana

  3. Center for Environmental Health, Toxicology and Management of Chemicals, CHE: 3328 Si Ayutthaya Road, Bangkok, 10400, Thailand

    Jitpisut Seepratoomrosh, Prayad Pokethitiyook, Metha Meetam & Wanvisa Pugkaew

  4. Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, NC, 27695, USA

    Jitpisut Seepratoomrosh, Wenqiao Yuan & Wanvisa Pugkaew

  5. PTT Research and Technology Institute, Phra Nakhon Si Ayutthaya, 13170, Thailand

    Kunn Kangvansaichol

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  1. Jitpisut Seepratoomrosh
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Correspondence to Prayad Pokethitiyook.

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Seepratoomrosh, J., Pokethitiyook, P., Meetam, M. et al. The Effect of Light Stress and Other Culture Conditions on Photoinhibition and Growth of Dunaliella tertiolecta . Appl Biochem Biotechnol 178, 396–407 (2016). https://doi.org/10.1007/s12010-015-1882-x

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  • DOI: https://doi.org/10.1007/s12010-015-1882-x

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