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A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide

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Abstract

Compared to Trichoderma reesei RUT-C30 cellulase (Trcel), Penicillium oxalicum 16 cellulase (P16cel) from the fermentation supernatant produced a 2-fold higher glucose yield when degrading microcrystalline cellulose (MCC), possessed a 10-fold higher β-glucosidase (BGL) activity, but obtained somewhat lower other cellulase component activities. The optimal temperature and pH of β-1,4-endoglucanase, cellobiohydrolase, and filter paperase from P16cel were 50–60 °C and 4–5, respectively, but those of BGL reached 70 °C and 5. The cellulase cocktail of P16cel and Trcel had a high synergism when solubilizing MCC and generated 1.7-fold and 6.2-fold higher glucose yields than P16cel and Trcel at the same filter paperase loading, respectively. Additional low concentration of fructose enhanced the glucose yield during enzymatic hydrolysis of MCC; however, additional high concentration of monosaccharide (especially glucose) reduced cellulase activities and gave a stronger monosaccharide inhibition on Trcel. These results indicate that P16cel is a more excellent cellulase than Trcel.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973, Program NO.2012CB721103), National High Technology Research and Development Program of China (863, Program NO.2012AA101806), National Natural Science Foundation of China (31360217), and Doctoral Starting up Foundation of Jiangxi Normal University (5451).

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Authors and Affiliations

  1. College of Life Science, Jiangxi Normal University, Nanchang, 330022, China

    Xi-hua Zhao & Bin Tong

  2. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China

    Wei Wang & Dong-zhi Wei

  3. Research Center for Biomass Energy, East China University of Science and Technology, Shanghai, 200237, China

    Su-ping Zhang

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  1. Xi-hua Zhao
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  2. Wei Wang
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Correspondence to Xi-hua Zhao or Dong-zhi Wei.

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Zhao, Xh., Wang, W., Tong, B. et al. A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide. Appl Biochem Biotechnol 178, 173–183 (2016). https://doi.org/10.1007/s12010-015-1866-x

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