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Lactic Acid and Biosurfactants Production from Residual Cellulose Films

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Abstract

The increasing amounts of residual cellulose films generated as wastes all over the world represent a big scale problem for the meat industry regarding to environmental and economic issues. The use of residual cellulose films as a feedstock of glucose-containing solutions by acid hydrolysis and further fermentation into lactic acid and biosurfactants was evaluated as a method to diminish and revalorize these wastes. Under a treatment consisting in sulfuric acid 6 % (v/v); reaction time 2 h; solid liquid ratio 9 g of film/100 mL of acid solution, and temperature 130 °C, 35 g/L of glucose and 49 % of solubilized film was obtained. From five lactic acid strains, Lactobacillus plantarum was the most suitable for metabolizing the glucose generated. The process was scaled up under optimized conditions in a 2-L bioreactor, producing 3.4 g/L of biomass, 18 g/L of lactic acid, and 15 units of surface tension reduction of a buffer phosphate solution. Around 50 % of the cellulose was degraded by the treatment applied, and the liqueurs generated were useful for an efficient production of lactic acid and biosurfactants using L. plantarum. Lactobacillus bacteria can efficiently utilize glucose from cellulose films hydrolysis without the need of clarification of the liqueurs.

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Acknowledgments

Authors are grateful to Programa de Mejoramiento del Profesorado (PROMEP) for the financial support of this work by the grant PROMEP/103.5/11/6945.

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Coordinación Académica Región Huasteca Sur, Universidad Autónoma de San Luis Potosí, Tamazunchale, San Luis Potosí, 79960, México

    Oscar Manuel Portilla Rivera

  2. Ingeniería Agroindustrial, Universidad Politécnica de Guanajuato, Cortázar, Guanajuato, 38483, México

    Guillermo Arzate Martínez & Lorenzo Jarquín Enríquez

  3. Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del Instituto Politécnico Nacional Unidad Querétaro, Cerro Blanco 141, Colinas del Cimatario, Querétaro, 76090, México

    Pedro Alberto Vázquez Landaverde

  4. Chemical Engineering Department, Faculty of Sciences, University of Vigo (Campus Ourense), Ourense, Spain and Agro-Food Biotechnology Laboratory, CITI—Research, Transfer and Innovation Centre, Technological Park of Galicia, San Cibrao das Viñas, Ourense, Spain

    José Manuel Domínguez González

Authors
  1. Oscar Manuel Portilla Rivera
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  2. Guillermo Arzate Martínez
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  3. Lorenzo Jarquín Enríquez
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Correspondence to Oscar Manuel Portilla Rivera or José Manuel Domínguez González.

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Portilla Rivera, O.M., Arzate Martínez, G., Jarquín Enríquez, L. et al. Lactic Acid and Biosurfactants Production from Residual Cellulose Films. Appl Biochem Biotechnol 177, 1099–1114 (2015). https://doi.org/10.1007/s12010-015-1799-4

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  • DOI: https://doi.org/10.1007/s12010-015-1799-4

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