Abstract
Hyaluronidases (HAases) from yeasts were characterized for the first time. The study elucidated that hyaluronate 4-glycanohydrolase and hyaluronan (HA) lyase can be produced by yeasts. Six yeasts producing HAases were found through express screening of activities. The extracellular HAases from two of the yeast isolates, Pseudozyma aphidis and Cryptococcus laurentii, were characterized among them. P. aphidis HAase hydrolyzed β-1,4 glycosidic bonds of HA, yielding even-numbered oligosaccharides with N-acetyl-d-glucosamine at the reducing end. C. laurentii produced hyaluronan lyase, which cleaved β-1,4 glycosidic bonds of HA in β-elimination reaction, and the products of HA degradation were different-sized even-numbered oligosaccharides. The shortest detected HA oligomer was dimer. The enzymes’ pH and temperature optima were pH 3.0 and 37–45 °C (P. aphidis) and pH 6.0 and 37 °C (C. laurentii), respectively. Both HAases showed good thermostability.
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Acknowledgments
We would like to thank Dr. Renata Vadkertiová from the Culture Collection of Yeasts, Institute of Chemistry, Slovak Academy of Science, for the consultative help and constructive suggestion on the selection of microorganisms for the screening.
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The authors declare that they have no competing interests.
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Smirnou, D., Krčmář, M., Kulhánek, J. et al. Characterization of Hyaluronan-Degrading Enzymes from Yeasts. Appl Biochem Biotechnol 177, 700–712 (2015). https://doi.org/10.1007/s12010-015-1774-0
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DOI: https://doi.org/10.1007/s12010-015-1774-0