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Mechanism of Adsorptive Removal of Methylene Blue Using Dried Biomass of Rhizopus oryzae

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Abstract

Adsorption is an efficient way to remove synthetic dyes from industrial effluent. Here, we show mechanism of adsorptive removal of cationic dye methylene blue (MB) from its aqueous solution using dried biomass of Rhizopus oryzae as a biosorbent. The optimum pH and temperature for adsorption was found to be 7.0 and 28 °C, respectively. Scanning electron microscopy (SEM) of the biomass suggested distinct changes in surface topology post-MB adsorption, while Fourier transform infrared (FTIR) study indicated chemical interaction between the surface of the biomass and MB. Chemical modification of –OH and –C=O groups of biomass reduced the MB adsorption and corroborated with the FTIR analyses. Kinetics study revealed that the adsorption rate was fast initially and reached equilibrium at 4 h following a pseudo-second-order-kinetics. The adsorption isotherm followed Freundlich isotherm model with n value of 1.1615.The dried biomass of R. oryzae can be used as a potent biosorbent for the removal of MB.

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Acknowledgments

The authors acknowledge the research support provided by Tezpur University, Tezpur. RM acknowledges funds received from the Department of Biotechnology Govt. of India and start-up funds from Tezpur University. Authors sincerely thank Dr. L.G. Roy, Jadavpur University, Kolkata, India for the fungal strain.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, 784028, India

    Manash Deep Dey, Ruchi Shukla, Naba K. Bordoloi, Robin Doley & Rupak Mukhopadhyay

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  1. Manash Deep Dey
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  2. Ruchi Shukla
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Correspondence to Rupak Mukhopadhyay.

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Dey, M.D., Shukla, R., Bordoloi, N.K. et al. Mechanism of Adsorptive Removal of Methylene Blue Using Dried Biomass of Rhizopus oryzae . Appl Biochem Biotechnol 177, 541–555 (2015). https://doi.org/10.1007/s12010-015-1761-5

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