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Expression of Finger Millet EcDehydrin7 in Transgenic Tobacco Confers Tolerance to Drought Stress

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Abstract

One of the critical alarming constraints for agriculture is water scarcity. In the current scenario, global warming due to climate change and unpredictable rainfall, drought is going to be a master player and possess a big threat to stagnating gene pool of staple food crops. So it is necessary to understand the mechanisms that enable the plants to cope with drought stress. In this study, effort was made to prospect the role of EcDehydrin7 protein from normalized cDNA library of drought tolerance finger millet in transgenic tobacco. Biochemical and molecular analyses of T0 transgenic plants were done for stress tolerance. Leaf disc assay, seed germination test, dehydration assay, and chlorophyll estimation showed EcDehydrin7 protein directly link to drought tolerance. Northern and qRT PCR analyses shows relatively high expression of EcDehydrin7 protein compare to wild type. T0 transgenic lines EcDehydrin7(11) and EcDehydrin7(15) shows superior expression among all lines under study. In summary, all results suggest that EcDehydrin7 protein has a remarkable role in drought tolerance and may be used for sustainable crop breeding program in other food crops.

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References

  1. Tenywa, J. S., Nyende, P., Kidoido, M., Kasenge, V., Oryokot, J., & Mbowa, S. (1999). Prospects and constraints of finger millet production in Eastern Uganda. African Crop Science Journal, 7, 569–583.

    Google Scholar 

  2. Tadele, Z. (2007). Role of orphan crops in enhancing and diversifying food production in Africa. African Technology Development Forum - Journal, 6, 9–15.

    Google Scholar 

  3. Rahman, H., Jagadeeshselvam, N., Valarmathi, R., Sachin, B., Sasikala, R., Senthil, N., Sudhakar, D., Robin, S., & Muthurajan, R. (2014). Transcriptome analysis of salinity responsiveness in contrasting genotypes of finger millet (Eleusine coracana L.) through RNA-sequencing. Plant Molecular Biology, 85, 485–503.

    Article  CAS  Google Scholar 

  4. Solomon, S. (ed). (2007). Climate change 2007—the physical science basis: Working group I contribution to the fourth assessment report of the IPCC (Vol. 4). Cambridge University Press.

  5. IPCC special report on carbon dioxide capture and storage, Chap. 5. http://www.ipcc.ch/pdf/special-reports/srccs/srccs_wholereport.pdf. Accessed 14 October 2008.

  6. Mittler, R., & Blumwald, E. (2010). Genetic engineering for modern agriculture: challenges and perspectives. Annual Review of Plant Biology, 61, 443–462.

    Article  CAS  Google Scholar 

  7. Boyer, J. S. (1982). Plant productivity and environment. Science, 218, 443–448.

    Article  CAS  Google Scholar 

  8. Bray, E. A., Bailey-Serres, J., & Weretilnyk, E. (2000). Responses to abiotic stress. In B. B. Buchanan, W. Gruissem, & R. L. Jones (Eds.), Biochemistry and Molecular Biology of Plants (pp. 1158–1203). Waldorf: American Society of Plant Biologists.

    Google Scholar 

  9. de Carvalho, M. H. C. (2008). Drought stress and reactive oxygen species. Plant Signal Behavior, 3, 156–165.

    Article  Google Scholar 

  10. Singh, R. K., Phanindra, M. L. V., Singh, V. K., Sonam, R. S., Solanke, A. U., & Kumar, P. A. (2014). Isolation and characterization of drought responsive EcDehydrin7 gene from finger millet (Eleusine coracana (L.) Gaertn.). Indian Journal of Genetics and Plant Breeding, 74, 456–462.

    Article  CAS  Google Scholar 

  11. Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Article  CAS  Google Scholar 

  12. Horsch, R. B., Fry, J. E., Hoffmann, N. L., Eichholtz, D., Rogers, S. A., & Fraley, R. T. (1985). A simple and general method for transferring genes into plants. Science, 227, 1229–1231.

    Article  CAS  Google Scholar 

  13. Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1–15.

    Article  CAS  Google Scholar 

  14. Mundy, J., & Chua, N. H. (1988). Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO Journal, 7, 2279–2286.

    CAS  Google Scholar 

  15. Dure, L. S. (1993). Structural motifs in LEA proteins. In E. A. Bray, & T. J. Close (Eds.), Plant responses to cellular dehydration during environmental stress (pp. 91–103). Rockville: The American Society of Plant Physiologist.

    Google Scholar 

  16. Close, T. J., Kortt, A. A., & Chandler, P. M. (1989). A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Molecular Biology, 13, 95–108.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are thankful to the Department of Biotechnology (DBT), Govt. of India, and Indian Council of Agricultural Research (ICAR) for financial assistance; and AICP on small millets, University of Agricultural Sciences, Bangalore, for providing seeds of finger millet.

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Author notes

  1. Polumetla Ananda Kumar

    Present address: Institute of Biotechnology, Acharya N.G. Ranga Agricultural University, Hyderabad, 500030, India

Authors and Affiliations

  1. National Research Centre on Plant Biotechnology, LBS Building, Pusa Campus, New Delhi, 110012, India

    Rajiv Kumar Singh, Vivek Kumar Singh, Sanagala Raghavendrarao, Mullapudi Lakshmi Venkata Phanindra, K. Venkat Raman, Amolkumar U. Solanke, Polumetla Ananda Kumar & Tilak Raj Sharma

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  1. Rajiv Kumar Singh
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  2. Vivek Kumar Singh
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  3. Sanagala Raghavendrarao
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  4. Mullapudi Lakshmi Venkata Phanindra
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  5. K. Venkat Raman
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  6. Amolkumar U. Solanke
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  7. Polumetla Ananda Kumar
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  8. Tilak Raj Sharma
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Correspondence to Polumetla Ananda Kumar or Tilak Raj Sharma.

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Singh, R.K., Singh, V.K., Raghavendrarao, S. et al. Expression of Finger Millet EcDehydrin7 in Transgenic Tobacco Confers Tolerance to Drought Stress. Appl Biochem Biotechnol 177, 207–216 (2015). https://doi.org/10.1007/s12010-015-1738-4

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  • DOI: https://doi.org/10.1007/s12010-015-1738-4

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