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Applied Biochemistry and Biotechnology

, Volume 177, Issue 1, pp 207–216 | Cite as

Expression of Finger Millet EcDehydrin7 in Transgenic Tobacco Confers Tolerance to Drought Stress

  • Rajiv Kumar Singh
  • Vivek Kumar Singh
  • Sanagala Raghavendrarao
  • Mullapudi Lakshmi Venkata Phanindra
  • K. Venkat Raman
  • Amolkumar U. Solanke
  • Polumetla Ananda KumarEmail author
  • Tilak Raj SharmaEmail author
Article

Abstract

One of the critical alarming constraints for agriculture is water scarcity. In the current scenario, global warming due to climate change and unpredictable rainfall, drought is going to be a master player and possess a big threat to stagnating gene pool of staple food crops. So it is necessary to understand the mechanisms that enable the plants to cope with drought stress. In this study, effort was made to prospect the role of EcDehydrin7 protein from normalized cDNA library of drought tolerance finger millet in transgenic tobacco. Biochemical and molecular analyses of T0 transgenic plants were done for stress tolerance. Leaf disc assay, seed germination test, dehydration assay, and chlorophyll estimation showed EcDehydrin7 protein directly link to drought tolerance. Northern and qRT PCR analyses shows relatively high expression of EcDehydrin7 protein compare to wild type. T0 transgenic lines EcDehydrin7(11) and EcDehydrin7(15) shows superior expression among all lines under study. In summary, all results suggest that EcDehydrin7 protein has a remarkable role in drought tolerance and may be used for sustainable crop breeding program in other food crops.

Keywords

Finger millet Drought EcDehydrin7 Transgenics and qRT PCR 

Notes

Acknowledgments

The authors are thankful to the Department of Biotechnology (DBT), Govt. of India, and Indian Council of Agricultural Research (ICAR) for financial assistance; and AICP on small millets, University of Agricultural Sciences, Bangalore, for providing seeds of finger millet.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Rajiv Kumar Singh
    • 1
  • Vivek Kumar Singh
    • 1
  • Sanagala Raghavendrarao
    • 1
  • Mullapudi Lakshmi Venkata Phanindra
    • 1
  • K. Venkat Raman
    • 1
  • Amolkumar U. Solanke
    • 1
  • Polumetla Ananda Kumar
    • 1
    • 2
    Email author
  • Tilak Raj Sharma
    • 1
    Email author
  1. 1.National Research Centre on Plant Biotechnology, LBS Building, Pusa CampusNew DelhiIndia
  2. 2.Institute of BiotechnologyAcharya N.G. Ranga Agricultural UniversityHyderabadIndia

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