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Applied Biochemistry and Biotechnology

, Volume 177, Issue 1, pp 175–189 | Cite as

Studies on Plant Growth Promoting Properties of Fruit-Associated Bacteria from Elettaria cardamomum and Molecular Analysis of ACC Deaminase Gene

  • B. Jasim
  • Mathew Chacko Anish
  • Vellakudiyan Shimil
  • Mathew Jyothis
  • E. K. RadhakrishnanEmail author
Article

Abstract

Endophytic microorganisms have been reported to have diverse plant growth promoting mechanisms including phosphate solubilization, N2 fixation, production of phyto-hormones and ACC (1-aminocyclopropane-1-carboxylate) deaminase and antiphyto-pathogenic properties. Among these, ACC deaminase production is very important because of its regulatory effect on ethylene which is a stress hormone with precise role in the control of fruit development and ripening. However, distribution of these properties among various endophytic bacteria associated with fruit tissue and its genetic basis is least investigated. In the current study, 11 endophytic bacteria were isolated and identified from the fruit tissue of Elettaria cardamomum and were studied in detail for various plant growth promoting properties especially ACC deaminase activity using both culture-based and PCR-based methods. PCR-based screening identified the isolates EcB 2 (Pantoea sp.), EcB 7 (Polaromonas sp.), EcB 9 (Pseudomonas sp.), EcB 10 (Pseudomonas sp.) and EcB 11 (Ralstonia sp.) as positive for ACC deaminase. The PCR products were further subjected to sequence analysis which proved the similarity of the sequences identified in the study with ACC deaminase sequences reported from other sources. The detailed bioinformatic analysis of the sequence including homology-based modelling and molecular docking confirmed the sequences to have ACC deaminase activity. The docking of the modelled proteins was done using patch dock, and the detailed scrutiny of the protein ligand interaction revealed conservation of key amino acids like Lys51, Ser78, Tyr268 and Tyr294 which play important role in the enzyme activity. These suggest the possible regulatory effect of these isolates on fruit physiology.

Keywords

Endophyte Plant growth promotion ACC deaminase Elettaria cardamomum In silico modelling and docking 

Notes

Acknowledgments

This study was supported by the Department of Biotechnology (DBT), Government of India under DBT-RGYI and DBT-MSUB schemes and Kerala State Council for Science, Technology & Environment (KSCSTE), Government of Kerala under KSCSTE-SARD support programme.

Ethical Standards

We have not used any animal models for the experiments and thus do not require ethical committee clearance.

Conflict of Interest

The authors declare no conflict of interest.

Supplementary material

12010_2015_1736_MOESM1_ESM.doc (1.2 mb)
Fig. S1 Validation of the 3D protein structure obtained by homology modelling of the protein sequence of the ACC deaminase gene using Ramachandran plot. a – Isolate EcB 2; b – Isolate ECB 7; c – Isolate ECB 9; d – Isolate ECB 10; e – Isolate ECB 11; f – Template (ACC deaminase gene of Pseudomonas sp. (strain ACP) AAA25689) (doc 1270 kb)
12010_2015_1736_MOESM2_ESM.docx (12 kb)
Table S1 (docx 11.7 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • B. Jasim
    • 1
  • Mathew Chacko Anish
    • 2
  • Vellakudiyan Shimil
    • 1
  • Mathew Jyothis
    • 1
  • E. K. Radhakrishnan
    • 1
    Email author
  1. 1.School of BiosciencesMahatma Gandhi UniversityKottayamIndia
  2. 2.Department of ZoologySt. Berchmans CollegeKottayamIndia

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