Characterization of an Antibacterial Compound, 2-Hydroxyl Indole-3-Propanamide, Produced by Lactic Acid Bacteria Isolated from Fermented Batter
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Lactic acid bacteria are known to produce numerous antimicrobial compounds that are active against various pathogens. Here, we have purified and characterized a novel low-molecular-weight (LMW) antimicrobial compound produced by Lactobacillus and Pediococcus isolated from fermented idly and uttapam batter. The LMW compound was extracted from cell-free supernatant using ice-cold acetone, purified by gel permeation and hydrophobic interaction chromatography. It exhibited antimicrobial activity against Gram-positive and Gram-negative pathogenic bacteria sparing the probiotic strains like Lactobacillus rhamnosus. The molecular weight of the LMW compound was identified as 204 Da using LC-MS-ESI. In addition, the structure of the compound was predicted using spectroscopic methods like FTIR and NMR and identified as 2-hydroxyl indole-3-propanamide. The LMW compound was differentiated from its related compound, tryptophan, by Salkowski reaction and thin-layer chromatography. This novel LMW compound, 2-hydroxyl indole-3-propanamide, may have an effective application as an antibiotic which can spare prevailing probiotic organisms but target only the pathogenic strains.
KeywordsLactobacillus Pediococcus Antibacterial NMR FTIR 2-Hydroxyl indole-3-propanamide
The authors are thankful to ER&IPR, Defense Research & Development Organization, New Delhi, for funding this project and Defence R&D Establishment, Gwalior, for providing facilities for structural elucidation of this compound. Ms. Jayaprabha Agaliya is thankful for providing fellowship in this project, while Ms. Saraniya and Mr. Vidhyasagar are thankful to University Grants Commission, New Delhi, for providing fellowship.
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