Abstract
In the present study, the antimicrobial properties of modified biopolymers such as gelatin and agar have been investigated. These biopolymers (agar and gelatin) are modified by dissolving in ionic liquid (IL) [1-ethyl-3-methylimidazolium chloride ([C2mim][Cl]) and 1-octyl-3-methyl imidazolium chloride ([C8mim][Cl])] solutions. It was noticed that agar-ionogel (Ag-IL), gelatin-ionogel (GB-IL), and gelatin organogel (gelatin-glycerol solution along with laponite, nanoclay) nanocomposite (GA-NC) formed are highly stable, optically clear, and transparent without any air bubbles. The antimicrobial activity of these (Ag-IL), (GB-IL), and GA-NC were analyzed for both gram-negative (Escherichia coli, Klebsiella pneumoniae) and gram-positive bacterial strains (Staphylococcus aureus and Staphylococcus pyogenes) and fungus A. niger, C. albicans. Antibacterial and antifungal activity studies were carried out at different dilutions such as 100, 99, and 90 % (v/v). It was found that Ag-IL, GB-IL, and individual IL ([C8mim][Cl]) exhibited superior antimicrobial activities, indicating that longer IL chain enhance the cell membrane permeability of S. aureus, S. pyogenes, and E. coli cells. However, GA-NC nanocomposite and [C2mim][Cl]-based composites does not exhibit any bacterial inhibition activity for all bacterial strains.
References
Petkovic, M., Seddon, K. R., Rebelo, L. P. N., & Silva Pereira, C. (2011). Ionic liquids: a pathway to environmental acceptability. Chemical Society Reviews, 40, 1383–1403.
Busetti, A., Crawford, D. E., Earle, M. J., Gilea, M. A., Gilmore, B. F., Gorman, S. P., Laverty, G., Lowry, A. F., McLaughlin, M., & Seddon, K. R. (2010). Antimicrobial and antibiofilm activities of 1-alkylquinolinium bromide ionic liquids. Green Chemistry, 12, 420–425.
Huddleston, J. G., Visser, A. E., Reichert, W. M., Willauer, H. D., Broker, G. A., & Rogers, R. D. (2001). Characterization and comparison of hydrophilic and hydrophobic room temperature ionic liquids incorporating the imidazolium cation. Green Chemistry, 3, 156–164.
Docherty, K. M., & Kulpa, J. C. F. (2005). Toxicity and antimicrobial activity of imidazolium and pyridinium ionic liquids. Green Chemistry, 7, 185–189.
Pernak, J., Sobaszkiewicz, K., & Mirska, I. (2003). Anti-microbial activities of ionic liquids. Green Chemistry, 5, 52–56.
Le Bideau, J., Viau, L., & Vioux, A. (2011). Ionogels, ionic liquid based hybrid materials. Chemical Society Reviews, 40, 907–925.
Trewyn, B. G., Whitman, C. M., & Lin, V. S. Y. (2004). Morphological control of room-temperature ionic liquid templated mesoporous silica nanoparticles for controlled release of antibacterial agents. Nano Letters, 4, 2139–2143.
Venkata Nancharaiah, Y., Reddy, G. K. K., Lalithamanasa, P., & Venugopalan, V. P. (2012). The ionic liquid 1-alkyl-3-methylimidazolium demonstrates comparable antimicrobial and antibiofilm behavior to a cationic surfactant. Biofouling, 28, 1141–1149.
Sharma, A., Rawat, K., Solanki, P. R., & Bohidar, H. B. (2015). Gelatin-ionic liquid based platform for glucose detection. Current Topics in Medicinal Chemistry, 15, 1257–1267.
Fujita, K., MacFarlane, D. R., Forsyth, M., Yoshizawa-Fujita, M., Murata, K., Nakamura, N., & Ohno, H. (2007). Biomacromolecules, 8, 2080–2086.
Rawat, K., Pathak, J., & Bohidar, H. B. (2014). Effect of solvent hydrophobicity on gelation kinetics and phase diagram of gelatin ionogels. Soft Matter, 10, 862–872.
He, Y., & Lodge, T. P.(2007). A thermoreversible ion gel by triblock copolymer self-assembly in an ionic liquid. Chemical Communications, 2732–2734.
Ji, X., Held, C., & Sadowski, G. (2014). Modeling imidazolium-based ionic liquids with ePC-SAFT. Part II. Application to H2S and synthesis-gas components. Fluid Phase Equilibria, 363, 59–65.
Rhim, J.-W. (2011). Effect of clay contents on mechanical and water vapor barrier properties of agar-based nanocomposite films. Carbohydrate Polymers, 86, 691–699.
Cha, D. S., & Chinnan, M. S. (2004). Biopolymer-based antimicrobial packaging: a review. Critical Reviews in Food Science and Nutrition, 44, 223–237.
Staphylococcal skin infections. http://dermnetnz.org/bacterial/staphylococci.html.
Streptococcal skin infections. http://www.dermnetnz.org/bacterial/streptococcal-disease.html.
Giordano, P., Weber, K., Gesin, G., & Kubert, J. (2007). Skin and skin structure infections: treatment with newer generation fluoroquinolones. Therapeutics and Clinical Risk Management, 3, 309–317.
Khalid, R., & Solan, M. Candida fungus skin infection, in, 2012. http://www.healthline.com/health/skin/candida-fungus#Overview1.
Aspergillosis. http://www.dermnetnz.org/fungal/aspergillosis.html.
(1978). Handbook of phycological methods: Physiological and biochemical methods. In J. A. Hellebust & J. S. Craigie (Eds.). Cambridge University Press. xi, 512 pp. Price £18.00, Journal of the Marine Biological Association of the United Kingdom, 59 (1979) 809-809.
Pujala, R. K., Pawar, N., & Bohidar, H. B. (2011). Universal sol state behavior and gelation kinetics in mixed clay dispersions. Langmuir, 27, 5193–5203.
Cornellas, A., Perez, L., Comelles, F., Ribosa, I., Manresa, A., & Garcia, M. T. (2011). Self-aggregation and antimicrobial activity of imidazolium and pyridinium based ionic liquids in aqueous solution. Journal of Colloid and Interface Science, 355, 164–171.
Acknowledgments
AS acknowledges University Grants Commission, Government of India for research fellowship. KR acknowledges receipt of DST Inspire Faculty Award. This work was supported by a grant received from Department of Science and Technology, Government of India and University Grants Commission for start-up grant.
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Sharma, A., Prakash, P., Rawat, K. et al. Antibacterial and Antifungal Activity of Biopolymers Modified with Ionic Liquid and Laponite. Appl Biochem Biotechnol 177, 267–277 (2015). https://doi.org/10.1007/s12010-015-1727-7
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DOI: https://doi.org/10.1007/s12010-015-1727-7