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Cloning and Genomic Organization of a Rhamnogalacturonase Gene from Locally Isolated Strain of Aspergillus niger

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Abstract

The rhg gene encoding a rhamnogalacturonase was isolated from the novel strain A1 of Aspergillus niger. It consists of an ORF of 1.505 kb encoding a putative protein of 446 amino acids with a predicted molecular mass of 47 kDa, belonging to the family 28 of glycosyl hydrolases. The nature and position of amino acids comprising the active site as well as the three-dimensional structure were well conserved between the A. niger CTM10548 and fungal rhamnogalacturonases. The coding region of the rhg gene is interrupted by three short introns of 56 (introns 1 and 3) and 52 (intron 2) bp in length. The comparison of the peptide sequence with A. niger rhg sequences revealed that the A1 rhg should be an endo-rhamnogalacturonases, more homologous to rhg A than rhg B A. niger known enzymes. The comparison of rhg nucleotide sequence from A. niger A1 with rhg A from A. niger shows several base changes. Most of these changes (59 %) are located at the third base of codons suggesting maintaining the same enzyme function. We used the rhamnogalacturonase A from Aspergillus aculeatus as a template to build a structural model of rhg A1 that adopted a right-handed parallel β-helix.

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Acknowledgments

With deep sadness, we dedicate this work to the Memory of Dr. Noomen Hadj Taeib, one of the co-authors who passed away before the release of this research paper. Lamia Jmal-Hammami and Mosbeh Dardouri are thanked for their technical help. We thank Professors Raja Mokdad-Gargouri and Hafedh Belghith for the fruitful discussion of scientific interpretations. This work was supported by Grants from the Ministry of Higher Education and Scientific Research, Tunisia.

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  1. Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les EucaryotesCentre de Biotechnologie de Sfax, University of Sfax, Route Sidi Mansour, BP 1177, 3018, Sfax, Tunisia

    Naourez Damak, Salma Abdeljalil, Noomen Hadj Taeib & Ali Gargouri

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  1. Naourez Damak
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  2. Salma Abdeljalil
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Damak, N., Abdeljalil, S., Taeib, N.H. et al. Cloning and Genomic Organization of a Rhamnogalacturonase Gene from Locally Isolated Strain of Aspergillus niger . Appl Biochem Biotechnol 176, 2314–2327 (2015). https://doi.org/10.1007/s12010-015-1720-1

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