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Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material

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Abstract

Biosynthesized silver nanoparticles (AgNPs) incorporated polycaprolactone (PCL) nanomembrane was prepared by electrospinning as a cost-effective nanocomposite for application as an antimicrobial agent against wound infection. The nanocomposite membrane was characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis and Scanning Electron microscopy (SEM). The hydrophilicity analysis of electrospun membranes as evaluated by water contact angle measurement showed the change of hydrophobicity of PCL to hydrophilic upon incorporation of silver nanoparticles. Better mechanical properties were also observed for PCL membrane due to the incorporation of silver nanoparticles and are highly supportive to explore its biomedical applications. Further antibacterial analysis of silver nanoparticle-incorporated PCL membrane against common wound pathogens coagulase-negative Staphylococcus epidermidis and Staphylococcus haemolyticus showed remarkable activity. As biosynthesized AgNPs are least explored for clinical applications, the current study is a promising cost-effective method to explore the development of silver nanoparticle-based electrospun nanocomposite to resist wound-associated infection.

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Acknowledgments

The authors gratefully acknowledge the Indian Council of Medical Research (ICMR), India, for the funded project on CoNS and Senior Research Fellowship to the author Roshmi Thomas. We also thank the Department of Biotechnology, Government of India, for DBT RGYI and DBT – MSUB – IPLSARE Programmes in School of Biosciences, Mahatma Gandhi University. In addition, we thank the Director, Centre for Nanoscience and Nanotechnology, for the help and support for electrospinning unit, contact angle measurement, and tensile testing and the Director, School of Chemical Sciences, Mahatma Gandhi University, Kottayam, Kerala, India, for SEM analysis of samples. We also thank the Director, School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala, India, for the help in XRD analysis.

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686 560, India

    Roshmi Thomas, K. R. Soumya, Jyothis Mathew & E. K. Radhakrishnan

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  1. Roshmi Thomas
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  2. K. R. Soumya
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  3. Jyothis Mathew
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  4. E. K. Radhakrishnan
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Correspondence to E. K. Radhakrishnan.

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Thomas, R., Soumya, K.R., Mathew, J. et al. Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material. Appl Biochem Biotechnol 176, 2213–2224 (2015). https://doi.org/10.1007/s12010-015-1709-9

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  • DOI: https://doi.org/10.1007/s12010-015-1709-9

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