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Enhanced Xylitol Production by Mutant Kluyveromyces marxianus 36907-FMEL1 Due to Improved Xylose Reductase Activity

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Abstract

A directed evolution and random mutagenesis were carried out with thermotolerant yeast Kluyveromyces marxianus ATCC 36907 for efficient xylitol production. The final selected strain, K. marxianus 36907-FMEL1, exhibited 120 and 39 % improvements of xylitol concentration and xylitol yield, respectively, as compared to the parental strain, K. marxianus ATCC 36907. According to enzymatic assays for xylose reductase (XR) activities, XR activity from K. marxianus 36907-FMEL1 was around twofold higher than that from the parental strain. Interestingly, the ratios of NADH-linked and NADPH-linked XR activities were highly changed from 1.92 to 1.30 when K. marxianus ATCC 36907 and K. marxianus 36907-FMEL1 were compared. As results of KmXYL1 genes sequencing, it was found that cysteine was substituted to tyrosine at position 36 after strain development which might cause enhanced XR activity from K. marxianus 36907-FMEL1.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2014R1A1A4A01004700). This work was supported by the 2012 Research Grant from Kangwon National University.

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Authors and Affiliations

  1. Department of Bioengineering and Technology, Kangwon National University, Chuncheon, 200-701, Republic of Korea

    Jin-Seong Kim, Jae-Bum Park, Seung-Won Jang & Suk-Jin Ha

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  1. Jin-Seong Kim
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  2. Jae-Bum Park
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  3. Seung-Won Jang
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  4. Suk-Jin Ha
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Correspondence to Suk-Jin Ha.

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Kim, JS., Park, JB., Jang, SW. et al. Enhanced Xylitol Production by Mutant Kluyveromyces marxianus 36907-FMEL1 Due to Improved Xylose Reductase Activity. Appl Biochem Biotechnol 176, 1975–1984 (2015). https://doi.org/10.1007/s12010-015-1694-z

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