Skip to main content
SpringerLink
Log in

Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers. It is produced by algae and some species of Azotobacter and Pseudomonas. This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter. Results showed that alginate production increased with increasing DOT from 1 to 5 %. The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT. At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration. On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Hay, I. D., Rehman, Z. U., Ghafoor, A., & Rehm, B. H. A. (2010). Journal of Chemical Technology and Biotechnology, 85(6), 752–759.

    Article  CAS  Google Scholar 

  2. Sabra, W., Zeng, A. P., & Deckwer, W. D. (2001). Applied Microbiology and Biotechnology, 56(3-4), 315–325.

    Article  CAS  Google Scholar 

  3. Sabra, W., Zeng, A. P., Lunsdorf, H., & Deckwer, W. D. (2000). Applied and Environmental Microbiology, 66(9), 4037–4044.

    Article  CAS  Google Scholar 

  4. Sabra, W., Zeng, A. P., Sabry, S., Omar, S., & Deckwer, W. D. (1999). Applied Microbiology and Biotechnology, 52(6), 773–780.

    Article  CAS  Google Scholar 

  5. Asami, K., Aritomi, T., Tan, Y. S., & Ohtaguchi, K. (2004). Journal of Chemical Engineering of Japan, 37(8), 1050–1055.

    Article  CAS  Google Scholar 

  6. Moreno, J., Vargas-Garcia, C., Lopez, M. J., & Sanchez-Serrano, G. (1999). Journal of Applied Microbiology, 86(3), 439–445.

    Article  CAS  Google Scholar 

  7. Reyes, C., Pena, C., & Galindo, E. (2003). Journal of Biotechnology, 105(1-2), 189–198.

    Article  CAS  Google Scholar 

  8. Segura, D., Guzmaan, J., & Espin, G. (2003). Applied Microbiology and Biotechnology, 63(2), 159–163.

    Article  CAS  Google Scholar 

  9. Priego-Jimenez, R., Pena, C., Ramirez, O. T., & Galindo, E. (2005). Biochemical Engineering Journal, 25(3), 187–193.

    Article  CAS  Google Scholar 

  10. Diaz-Barrera, A., Silva, P., Berrios, J., & Acevedo, F. (2010). Bioresource Technology, 101(23), 9405–9408.

    Article  CAS  Google Scholar 

  11. Ertesvåg, H., Valla, S., & Skjåk-Bræk, G. (2009). In B. H. A. Rehm (Ed.), Alginates: biology and applications, Enzymatic alginate modification, alginates: biology and applications (pp. 95–115). Berlin: Springer-Verlag.

    Google Scholar 

  12. Annison, G., & Couperwhite, I. (1984). Applied Microbiology and Biotechnology, 19(5), 321–325.

    Article  CAS  Google Scholar 

  13. Annison, G., & Couperwhite, I. (1986). Applied Microbiology and Biotechnology, 25(1), 55–61.

    Article  CAS  Google Scholar 

  14. Ertesvag, H., Hoidal, H. K., Skjak-Braek, G., & Valla, S. (1998). Journal of Biological Chemistry, 273(47), 30927–30932.

    Article  CAS  Google Scholar 

  15. Ramstad, M. V., Markussen, S., Ellingsen, T. E., Skjak-Braek, G., & Levine, D. W. (2001). Enzyme and Microbial Technology, 28(1), 57–69.

    Article  Google Scholar 

  16. Hoidal, H. K., Ertesvag, H., Skjak-Braek, G., Stokke, B. T., & Valla, S. (1999). Journal of Biological Chemistry, 274(18), 12316–12322.

    Article  CAS  Google Scholar 

  17. Moral, C. K., & Sanin, F. D. (2012). Journal of Industrial Microbiology & Biotechnology, 39(3), 513–519.

    Article  Google Scholar 

  18. Miller, G. (1959). Analytical Chemistry, 31, 426–428.

    Article  CAS  Google Scholar 

  19. ASTM (2003) Standard test method for determining the chemical composition and sequence in alginate by proton nuclear magnetic resonance (1H NMR) spectroscopy. ASTM F 2259-03 2003.

  20. Moral, C. K., Dogan, O., & Sanin, F. D. (2013). Journal of Polymer Engineering, 33(3), 239–246.

    CAS  Google Scholar 

  21. Pena, C., Trujillo-Roldan, M. A., & Galindo, E. (2000). Enzyme and Microbial Technology, 27(6), 390–398.

    Article  CAS  Google Scholar 

  22. Parente, E., Crudele, M. A., Aquino, M., & Clementi, F. (1998). Journal of Industrial Microbiology & Biotechnology, 20(3-4), 171–176.

    Article  CAS  Google Scholar 

  23. Remminghorst, U., & Rehm, B. H. A. (2006). Biotechnology Letters, 28(21), 1701–1712.

    Article  CAS  Google Scholar 

  24. Moral Kıvılcımdan, Ç. (2011). PhD Thesis, Ankara: Middle East Technical University.

  25. Diaz-Barrera, A., Aguirre, A., Berrios, J., & Acevedo, F. (2011). Process Biochemistry, 46(9), 1879–1883.

    Article  CAS  Google Scholar 

  26. Savalgi, V., & Savalgi, V. (1992). Journal of General and Applied Microbiology, 38(6), 641–645.

    Article  Google Scholar 

  27. Obika, H., Sakakibara, J., & Kobayashi, Y. (1993). Bioscience, Biotechnology, and Biochemistry, 57(2), 332–333.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study is funded by the Turkish Prime Ministry-State Planning Organization and Middle East Technical University Research Fund (BAP-DPT 2002 K120510).

Author information

Authors and Affiliations

  1. Department of Environmental Engineering, Middle East Technical University, 06800, Ankara, Turkey

    Çiğdem Kıvılcımdan Moral & Faika Dilek Sanin

  2. Department of Environmental Engineering, Akdeniz University, 07058, Antalya, Turkey

    Çiğdem Kıvılcımdan Moral

  3. Department of Chemistry, Middle East Technical University, 06800, Ankara, Turkey

    Özdemir Doğan

Authors
  1. Çiğdem Kıvılcımdan Moral
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Özdemir Doğan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Faika Dilek Sanin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Faika Dilek Sanin.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kıvılcımdan Moral, Ç., Doğan, Ö. & Sanin, F.D. Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate. Appl Biochem Biotechnol 176, 875–891 (2015). https://doi.org/10.1007/s12010-015-1617-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-015-1617-z

Keywords

Search

Navigation