Abstract
This work shows new and promising experimental data of soybean oil and canola oil glycerolysis using Novozym 435 enzyme as catalyst in a solvent-free system using ultrasound bath for the emulsifier, monoglyceride (MAG), and diacylglycerol (DAG) production. The experiments were conducted in batch mode to study the influence of process variables as temperature (40 to 70 °C), immobilized enzyme content (2.5 to 10 wt%, relative to substrates), molar ratio glycerol/oil (0.8:1 to 3:1), agitation (0 to 1200 rpm) and ultrasound intensity (0 to 132 W cm−2). Highest yields of DAG+MAG (75 wt%) were obtained with molar ratio glycerol/canola oil 0.8:1, 70 °C, 900 rpm, 120 min of reaction time, 10 wt% of enzyme concentration, and 52.8 W cm−2 of ultrasound intensity. When soybean oil was used, the best results in terms of DAG+MAGs (65 wt%) were using molar ratio of glycerol/soybean oil 0.8:1, 70 °C, 900 rpm, 90 min of reaction time, 10 wt% of enzyme content, and 40 % of ultrasound intensity (52.8 W cm−2). The results showed that the lipase-catalyzed glycerolysis in a solvent-free system with ultrasound bath can be a potential route for high content production of DAGs and MAGs.
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Xu, X. (2004). Nutritionally enhanced edible oil and oil seed processing. Champaign: AOCS Press.
Feltes, M. M. C., Pitol, L. O., Correia, J. F. G., Grimaldi, R., Block, J. M., & Ninow, J. L. (2009). Grasas y Aceites, 60, 168–176.
Rendón, X., López-Munguía, A., & Castillo, E. (2001). Journal of the American Oil Chemists Society, 78, 1061–1066.
Ferreira-Dias, S., Correia, A. C., Baptista, F. O., & Fonseca, M. M. R. (2001). Journal of Molecular Catalysis B: Enzymatic, 11, 699–711.
Damstrup, M. L., Jensen, T., Sparso, F. V., Kiil, S. Z., Jensen, A. D., & Xu, X. (2005). Journal of the American Oil Chemists’ Society, 82, 559–564.
Cheirsilp, B., Kaewthong, W., & H-Kittikun, A. (2007). Biochemical Engineering Journal, 35, 71–80.
Cheong, L. Z., Tan, C. P., Long, K., Yussof, M. S. A., Arifin, N., Lo, S. K., & Lai, O. M. (2007). Food Chemistry, 105, 1614–1622.
Kondo, H., Hase, T., Murase, T., & Tokimitsu, I. (2003). Lipids, 38, 25–30.
Chew, Y. H., Lee, C. T., Sarmidi, M. R., Aziz, R. A., & Razali, F. (2008). Food and Bioproducts Processing, 86, 267–282.
Voll, J. C., & Brito, M. N. (2010). Revista Saúde e Pesquisa, 3, 121–126.
Vulfson, E. N., Sarney, D. B., & Law, B. A. (1991). Enzyme and Microbial Technology, 13, 123–126.
Yachmenev, V. G., Blanchard, E. J., & Lambert, A. H. (2004). Ultrasonics, 42, 87–91.
Yoshimoto, M., Li, C., Ogata, H., Tsukuda, N., Fukunaga, K., & Nakao, K. (2005). Ultrasonics Sonochemistry, 12, 373–384.
Ma, H., Huang, L., Jia, J., He, R., Luo, L., & Zhu, W. (2011). Ultrasonics Sonochemistry, 18, 419–424.
Wang, Z., Lin, L., Li, P., Zhang, J., Wang, S., & Ma, H. (2012). Bioresource Technology, 117, 222–227.
Bashari, M., Eibaid, A., Wang, J., Tian, Y., Xu, X., & Jin, Z. (2012). Ultrasonics Sonochemistry, 20, 155–16.
Guiseppi-Eliea, S., Choi, K., & Geckelera, K. (2009). Journal of Molecular Catalysis B: Enzymatic, 58, 118–123.
Jian, S., Wenyi, T., & Wuyong, C. (2008). Journal of Cleaner Products, 16, 591–597.
Lee, S. H., Nguyen, H. M., Koo, Y. M., & Ha, S. H. (2008). Process Biochemistry, 43, 1009–1012.
Fiametti, K. G., Sychoski, M. M., Cesaro, A., Furigo, A. J., Bretanha, L. C., Pereira, C. M. P., Treichel, H., Oliveira, D., & Oliveira, J. V. (2011). Ultrasonics Sonochemistry, 18, 981–987.
Krüger, R. L., Valério, A., Balen, M., Ninow, J. L., De Oliveira, J. V., De Oliveira, D., & Corazza, M. L. (2010). European Journal of Lipid Science and Technology, 112, 921–927.
Liu, N., Wang, Y., Zhao, Q., Cui, C., Fu, M., & Zhao, M. (2012). Food Chemistry, 134, 301–307.
Kaewthong, W., & H-Kittikun, A. (2004). Enzyme and Microbial Technology, 35, 218–222.
Valério, A., Krüger, R. L., Ninow, J. L., Corazza, F. C., de Oliveira, D., de Oliveira, J. V., & Corazza, M. L. (2009). Journal of Agricultural and Food Chemistry, 57, 8350–8356.
Valério, A., Rovani, S., Treichel, H., Oliveira, D., & Vladimir, J. V. (2010). Bioprocess and Biosystems Engineering, 33, 805–812.
Dramstrup, M. L., Jensen, T., Sparso, F. V., Kiil, S. Z., Jensen, A. D., & Xu, X. (2006). Journal of the American Oil Chemists’ Society, 83, 27–33.
Antczak, M. S., Kubiak, A., Antczak, T., & Bielecki, S. (2009). Renewable Energy, 34, 1185–1194.
Zhong, N., Li, L., Xu, L., Cheong, X., Zhao, X., & Li, B. (2010). Food Chemistry, 122, 228–232.
Kristensen, J. B., Xu, X., & Mu, H. (2005). Journal of Agricultural and Food Chemistry, 53, 7059–7064.
Pawongrat, R., Xu, X., & H-Kittikun, A. (2007). Food Chemistry, 104, 251–258.
Wang, F., Zhang, H., Wang, J., Chen, G., Fang, X., Wang, Z., & Wang, L. (2012). Molecules, 17, 1–11.
Feltes, M. M. C., Oliveira, D., Block, J. M., & Ninow, J. L. (2012). Food and Bioprocess Technology, 6, 17–35.
Fiametti, K. G., Ustra, M. K., Oliveira, D., Corazza, M. L., Furigo, A. J., & Oliveira, J. V. (2012). Ultrasonics Sonochemistry, 19, 440–451.
Hoshino, Y., Kawasaki, T., & Okahata, Y. (2006). Biomacromolecules, 7, 682–685.
Kadkhodaee, R., & Povey, M. J. W. (2008). Ultrasonics Sonochemistry, 15, 133–142.
Löning, J. M., Horst, C., & Hoffmann, U. (2012). Ultrasonics Sonochemistry, 9, 169–179.
Lerin, L. A., Loss, R. A., Remonatto, D., Zenevicz, M. C., Balen, M., Netto, V. O., Ninow, J. L., Trentin, C. M., Oliveira, J. V., & Oliveira, D. (2014). Bioprocess and Biosystems Engineering, 37, 2381–2394.
Gonçalves, K. M., Sutili, F. K., Leite, S. G. F., de Souza, R. O. M. A., & Leal, I. C. R. (2012). Ultrasonics Sonochemistry, 19, 232–236.
Michelin, S., Penha, F. M., Sychoski, M. M., Scherer, R. P., Treichel, H., Valério, A., Di Luccio, M., Oliveira, D., & Oliveira, J. V. (2015). Renewable Energy, 76, 388–393.
Liu, S., Dong, X., Wei, F., Wang, X., Lv, X., Zhong, J., Wu, L., Quek, S., & Chen, H. (2015). Ultrasonics Sonochemistry, 23, 100–108.
Awadallak, J. A., Voll, F., Ribasa, M. C., Silva, C., Cardozo, F. L., & Silva, E. A. (2013). Ultrasonics Sonochemistry, 20, 1002–1007.
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The authors would like to thank CNPq and CAPES for the financial support for this research and also scholarships.
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Remonatto, D., Santin, C.M.T., Valério, A. et al. Lipase-Catalyzed Glycerolysis of Soybean and Canola Oils in a Free Organic Solvent System Assisted by Ultrasound. Appl Biochem Biotechnol 176, 850–862 (2015). https://doi.org/10.1007/s12010-015-1615-1
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DOI: https://doi.org/10.1007/s12010-015-1615-1