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Characterization of a Potential β-Lactamase Inhibitory Metabolite from a Marine Streptomyces sp. PM49 Active Against Multidrug-Resistant Pathogens

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Abstract

Actinobacteria is a prolific producer of complex natural products; we isolated a potential marine Streptomyces sp. PM49 strain from Bay of Bengal coastal area of India. The strain PM49 exhibited highly efficient antibacterial properties on multidrug-resistant pathogens with a zone of inhibition of 14–17 mm. SSF was adopted for the production of the secondary metabolites from PM49 with ISP2; utilizing agricultural wastes for compound extraction was also attempted. Bioactive fraction of Rf value 0.69 resolved using chloroform and ethyl acetate (1:1, v/v) was obtained and subjected to further analysis. Based on UV, IR, ESI-MS, and 1H and 13C NMR spectral analysis, it was revealed that the compound is closely similar to cyslabdan with a molecular mass of 467.66 corresponding to the molecular formula C25H41NO5S. ESBL and MBL production was screened in the hospital test isolates of Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, and Staphylococcus aureus. PCR amplification in the phenotypically positive strains was positive for bla IMP, bla SHV, bla CTX-M, and mec genes. The β-lactamase enzyme from tested strains had cephalosporinase activity with a 31-kDa protein and isolated compound from the strain possessing β-lactamase inhibitory potential. MIC of the active fraction was 16–32 μg/ml on ATCC strains; the ceftazidime and meropenem sensitive and resistant test strains showed MIC of 64–256 μg/ml. The Streptomyces sp. PM49 aerial mycelium was rectiflexibile; the 16S rRNA showed 99 % identity with Streptomyces rochei and submitted at Genbank with accession no JX904061.1.

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Acknowledgments

The authors are indebted to the microbiology staff members and physicians of the tertiary care hospital, Bangalore, for helping us in collecting the samples and providing ATCC strains, and they also acknowledge the technicians for their help. The authors also thank the Vice-Chancellor and Registrar of Periyar University, Salem, for their support and encouragement. This work was supported by the Indian Council of Medical Research [ICMR] New Delhi, India (ICMR letter no. 5/8/5/24/9/2011—ECD-I, dt.19.12.11).

Conflict of Interest

None declared.

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Authors and Affiliations

  1. Department of Microbiology, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India

    J. Shanthi

  2. Deparment of Bioengineering and Drug Design, Indian Institute of Technology, Chennai, 600036, Tamil Nadu, India

    A. Senthil

  3. Department of Microbiology, Periyar University, Salem, 636011, Tamil Nadu, India

    V. Gopikrishnan & R. Balagurunathan

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  1. J. Shanthi
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Correspondence to R. Balagurunathan.

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Shanthi, J., Senthil, A., Gopikrishnan, V. et al. Characterization of a Potential β-Lactamase Inhibitory Metabolite from a Marine Streptomyces sp. PM49 Active Against Multidrug-Resistant Pathogens. Appl Biochem Biotechnol 175, 3696–3708 (2015). https://doi.org/10.1007/s12010-015-1538-x

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