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Enzymatic Hydrolyzed Feather Peptide, a Welcoming Drug for Multiple-Antibiotic-Resistant Staphylococcus aureus: Structural Analysis and Characterization

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Abstract

This study aimed to explore the bactericidal activity of a feather-degraded active peptide against multiple-antibiotic-resistant (MAR) Staphylococcus aureus. An antibacterial peptide (ABP) was isolated from the chicken feathers containing fermented media of Paenibacillus woosongensis TKB2, a keratinolytic soil isolate. It was purified by HPLC, and its mass was found to be 4666.87 Da using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) spectroscopy. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of this peptide were 22.5 and 90 μg/ml, respectively. SEM study revealed the distorted cell wall of the test strain along with pore formation. The possible reason for bactericidal activity of the peptide is due to generation of reactive oxygen species (ROS), resulting in membrane damage and leakage of intracellular protein. Complete sequence of the peptide was predicted and retrieved from the sequence database of chicken feather keratin after in silico trypsin digestion using ExPASy tools. Further, net charge, hydrophobicity (77.7 %) and molecular modelling of the peptide were evaluated for better understanding of its mode of action. The hydrophobic region (17 to 27) of the peptide may facilitate for initial attachment on the bacterial membrane. The ABP exhibited no adverse effects on RBC membrane and HT-29 human cell line. This cytosafe peptide can be exploited as an effective therapeutic agent to combat Staphylococcal infections.

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Acknowledgments

Authors are thankful to the University Grant Commission [Sanction No: F.11-114/2008 (BSR)], Govt. of India, India, for the financial contribution in this study. We are also thankful to Mrs. Pratishruti Mandal, Assistant Teacher, Gokulpur Bidhan Chandra Vidyabhaban (H.S.), Gokulpur, Midnapore, West Bengal, India, for grammatical revision of this paper.

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Authors and Affiliations

  1. Department of Microbiology, Vidyasagar University, Midnapore, 721102,, West Bengal, India

    Tanmay Paul, Arpita Mandal, Santi M. Mandal, Kuntal Ghosh, Amit K. Mandal, Suman K. Halder, Arpan Das, Pradeep K. Das Mohapatra, Bikas R. Pati & Keshab C. Mondal

  2. Nilratan Sarkar Medical College and Hospital (NRS), Kolkata, West Bengal, India

    Suman K. Maji

  3. Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Kayisdagi, 34755, Istanbul, Turkey

    Ahmet Kati

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  1. Tanmay Paul
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Correspondence to Keshab C. Mondal.

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Paul, T., Mandal, A., Mandal, S.M. et al. Enzymatic Hydrolyzed Feather Peptide, a Welcoming Drug for Multiple-Antibiotic-Resistant Staphylococcus aureus: Structural Analysis and Characterization. Appl Biochem Biotechnol 175, 3371–3386 (2015). https://doi.org/10.1007/s12010-015-1509-2

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