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Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Laccase in Rhamnolipid Reversed Micellar System

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Abstract

Rhamnolipid was applied to degrade anthracene and pyrene in reversed micelles. The parameters in degradation were optimized for the purpose of improving degradation rates. The proper amount of rhamnolipid (RL) used for degrading anthracene was 0.065 mM, while 0.075 mM for pyrene. However, reaction time for degrading both anthracene and pyrene was 48 h. The optimum water content, pH, laccase concentration, polycyclic aromatic hydrocarbon (PAH) initial concentration, and volume ratio of n-hexanol to isooctane for both were found out. The highest degradation rates of anthracene and pyrene were 37.52 and 25.58 %, respectively. Although the degradation rates were not higher than the results previous literatures reported, this method was of novelty and provided guidance in application in degrading PAHs by reversed micellar system, especially for biosurfactant-based reversed micelles.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (21276069 and 71431006). The authors appreciate contributions of Kaitlin Jorgensen for language reviewing.

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People’s Republic of China

    Xin Peng, Xing-zhong Yuan, Huan Liu & Guang-ming Zeng

  2. Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha, 410082, People’s Republic of China

    Xin Peng, Xing-zhong Yuan, Huan Liu & Guang-ming Zeng

  3. Earth and Environmental Engineering, Columbia University, 500 West 120th Street, 937 Mudd Building, New York, NY, 10027, USA

    Xin Peng

  4. School of Business, Central South University, Changsha, 410083, People’s Republic of China

    Xiao-hong Chen

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  1. Xin Peng
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Correspondence to Xing-zhong Yuan.

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Peng, X., Yuan, Xz., Liu, H. et al. Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Laccase in Rhamnolipid Reversed Micellar System. Appl Biochem Biotechnol 176, 45–55 (2015). https://doi.org/10.1007/s12010-015-1508-3

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  • DOI: https://doi.org/10.1007/s12010-015-1508-3

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