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Isolation and Characterization of Cr(VI)-Reducing Actinomycetes from Estuarine Sediments

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Abstract

Bioremediation technologies have strong potential use in the less costly and more environmentally friendly removal of highly toxic hexavalent-chromium (Cr(VI)) compared with physicochemical technologies. Several Cr(VI)-reducing bacteria have been isolated; however, there are few studies on Cr(VI)-resistant and Cr(VI)-reducing actinomycetes. In this study, Cr(VI)-reducing actinomycetes were screened from estuarine, marine, and terrestrial samples on the basis of Cr(VI)-resistant and Cr(VI)-reducing ability. Of the 80 Streptomyces-like strains isolated, 20 strains were found to be resistant to 50 mg/l of Cr(VI). In addition, two strains isolated from the estuarine sediment of Tokyo Bay were found to be resistant to a concentration of 150 mg/l of Cr(VI). Furthermore, one Cr(VI)-reducing strain was found to remove 60 mg/l of Cr(VI) within 1 week and was identified as Streptomyces thermocarboxydus based on 16S rRNA gene analysis. The comparative evaluation with the type strain S. thermocarboxydus NBRC 16323 showed that our isolated strain had higher ability to grow at 27 °C and reduce Cr(VI) at a NaCl concentration of 6.0 % at 27 °C compared with the type strain NBRC 16323. These results indicate that our isolated strain have a potential ability to remove Cr(VI) from contaminated, highly saline sources without heating.

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Acknowledgments

We thank the officers and crew members of the research and training vessel “Hiyodori” of Tokyo University of Marine Science and Technology and the members of the International Coastal Research Center, Atmosphere and Ocean Research Institute, University of Tokyo, for supporting sample collection for this study. This research was supported in part by the Grant-in-Aid from the Faculty of Marine Science, Tokyo Universityof Marine Science and Technology and by the Cooperative Program of Atmosphere and Ocean Research Institute at the University of Tokyo.

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Authors and Affiliations

  1. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo, 108-8477, Japan

    Takeshi Terahara, Xudan Xu, Takeshi Kobayashi & Chiaki Imada

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  1. Takeshi Terahara
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  2. Xudan Xu
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  3. Takeshi Kobayashi
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  4. Chiaki Imada
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Correspondence to Takeshi Terahara.

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Terahara, T., Xu, X., Kobayashi, T. et al. Isolation and Characterization of Cr(VI)-Reducing Actinomycetes from Estuarine Sediments. Appl Biochem Biotechnol 175, 3297–3309 (2015). https://doi.org/10.1007/s12010-015-1501-x

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  • DOI: https://doi.org/10.1007/s12010-015-1501-x

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