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Kinetic Study of Candida antarctica Lipase B Immobilization Using Poly(Methyl Methacrylate) Nanoparticles Obtained by Miniemulsion Polymerization as Support

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Abstract

With the objective to obtain immobilized Candida antarctica lipase B (CalB) with good activity and improved utilization rate, this study evaluated the influence of enzyme and crodamol concentrations and initiator type on the CalB enzyme immobilization in nanoparticles consisting of poly(methyl methacrylate) (PMMA) obtained by miniemulsion polymerization. The kinetic study of immobilized CalB enzyme in PMMA nanoparticles was evaluated in terms of monomer conversion, particle size, zeta potential, and relative activity. The optimum immobilization condition for CalB was compared with free enzyme in the p-NPL hydrolysis activity measurement. Results showed a higher CalB enzyme stability after 20 hydrolysis cycles compared with free CalB enzyme; in particular, the relative immobilized enzyme activity was maintained up to 40 %. In conclusion, PMMA nanoparticles proved to be a good support for the CalB enzyme immobilization and may be used as a feasible alternative catalyst in industrial processes.

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Acknowledgments

The authors thank the financial support from CNPq—Conselho Nacional de Desenvolvimento Científico e Tecnológico and CAPES—Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, and Laboratório Central de Microscopia Eletrônica (LCME) of Federal University of Santa Catarina for TEM analyses.

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Authors and Affiliations

  1. Chemical Engineering and Food Engineering Department, Federal University of Santa Catarina (UFSC), P.O. Box 476, Florianópolis, SC, 88040-900, Brazil

    Alexsandra Valério, Gabrieli Nicoletti, Eliane P. Cipolatti, Jorge L. Ninow, Pedro H. H. Araújo, Cláudia Sayer & Débora de Oliveira

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  1. Alexsandra Valério
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  2. Gabrieli Nicoletti
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  3. Eliane P. Cipolatti
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  4. Jorge L. Ninow
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  5. Pedro H. H. Araújo
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  6. Cláudia Sayer
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  7. Débora de Oliveira
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Correspondence to Débora de Oliveira.

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Valério, A., Nicoletti, G., Cipolatti, E.P. et al. Kinetic Study of Candida antarctica Lipase B Immobilization Using Poly(Methyl Methacrylate) Nanoparticles Obtained by Miniemulsion Polymerization as Support. Appl Biochem Biotechnol 175, 2961–2971 (2015). https://doi.org/10.1007/s12010-015-1478-5

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  • DOI: https://doi.org/10.1007/s12010-015-1478-5

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