Skip to main content
SpringerLink
Log in

Comparison of Two Different Reactive Dye Immobilized Poly(Hydroxyethyl Methacrylate) Cryogel Discs for Purification of Lysozyme

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

In this study, cibacron blue F3GA and alkali blue 6B immobilized poly(hydroxyethyl methacrylate) [PHEMA] cryogel discs were prepared. The cryogel discs were characterised by swelling tests, elemental analysis and scanning electron microscopy. Each cryogel disc was used for lysozyme adsorption from aqueous solutions. Maximum adsorption capacities were 103.3 and 106.7 mg/g for cibacron blue F3GA and alkali blue 6B immobilized cryogel discs, respectively. Equilibrium lysozyme concentration, pH, ionic strength and temperature were the factors of which effect on lysozyme adsorption was investigated. Reusability of the cryogel discs was tested and less than 5 % decrease in adsorption capacity was reported. In the last stage of this work, the cryogel discs were used for lysozyme purification from chicken egg white. Molecular weight and purity of the eluted lysozyme from cryogel discs were controlled by sodium dodecyl sulfate polyacrylamide gel electrophoresis. In conclusion, cibacron blue F3GA and alkali blue 6B immobilized cryogel discs present a cheap and fast way for purification of lysozyme with a high purity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Callewaert, L., & Michiels, C. W. (2010). Journal of Biosciences, 35, 127–160.

    Article  CAS  Google Scholar 

  2. Callewaert, L., Van Herreweghe, J. M., Vanderkelen, L., Leysen, S., Voet, A., & Michiels, C. W. (2012). Trends in Microbiology, 20, 501–510.

    Article  CAS  Google Scholar 

  3. Van Herreweghe, J. M., & Michiels, C. W. (2012). Journal of Biosciences, 37, 327–348.

    Article  CAS  Google Scholar 

  4. Ghosh, R., & Cui, Z. F. (2000). Journal of Membrane Science, 167, 47–53.

    Article  CAS  Google Scholar 

  5. Juneja, V. K., Dwivedi, H. P., & Yan, X. (2012). Food Science and Technology, 3, 381–403.

    CAS  Google Scholar 

  6. Hartono, Y. D., Lee, A. N., Lee-Huang, S., & Zhang, D. (2011). Bioorganic & Medicinal Chemistry Letters, 21, 1607–1611.

    Article  CAS  Google Scholar 

  7. Odabası, M., & Denizli, A. (2004). Journal of Applied Polymer Science, 93, 719–725.

    Article  Google Scholar 

  8. Safarik, I., & Safarikova, M. (1993). Journal of Biochemical and Biophysical Methods, 27, 327–330.

    Article  CAS  Google Scholar 

  9. Chang, H. M., Yang, C. C., & Chang, Y. C. (2000). Journal of Agricultural and Food Chemistry, 48, 161–164.

    Article  CAS  Google Scholar 

  10. Wan, Y. H., Lu, J. R., & Cui, Z. F. (2006). Separation and Purification Technology, 48, 133–142.

    Article  CAS  Google Scholar 

  11. Sun, J., Su, Y., Rao, S., & Yang, Y. (2011). Journal of Chromatography B, 879, 2194–2200.

    Article  CAS  Google Scholar 

  12. Grasselli, M., Camperi, S. A., Navarro del Cañizo, A. A., & Cascone, O. (1999). Journal of the Science of Food and Agriculture, 79, 333–339.

    Article  CAS  Google Scholar 

  13. Uygun, D. A., Karagözler, A. A., Akgöl, S., & Denizli, A. (2009). Materials Science and Engineering: C, 29, 2165–2173.

    Article  Google Scholar 

  14. Wolman, F. J., Copello, G. J., Mebert, A. M., Targovnik, A. M., Miranda, M. V., Navarro del Cañizo, A. A., Díaz, L. E., & Cascone, O. (2010). European Food Research and Technology, 231, 181–188.

    Article  CAS  Google Scholar 

  15. Denizli, A., Salih, B., Kozluca, A., & Pişkin, E. J. (1997). Biomaterials Science, Polymer Edition, 8, 411–420.

    Article  CAS  Google Scholar 

  16. Denizli, A., & Pişkin, E. (2001). Journal of Biochemical and Biophysical Methods, 49, 391–416.

    Article  CAS  Google Scholar 

  17. Altıntaş, E. B., & Denizli, A. (2006). International Journal of Biological Macromolecules, 38, 99–106.

    Article  Google Scholar 

  18. Li, Z., Cao, M., Zhang, W., Liu, L., Wang, J., Ge, W., Yuan, Y., Yue, T., Li, R., & Yu, W. W. (2014). Food Chemistry, 145, 749–755.

    Article  CAS  Google Scholar 

  19. Plieva, F. M., Ekström, P., Galaev, I. Y., & Mattiasson, B. (2008). Soft Matter, 4, 2418–2428.

    Article  CAS  Google Scholar 

  20. Bereli, N., Andac, M., Baydemir, G., Say, R., Galaev, I. Y., & Denizli, A. (2008). Journal of Chromatography. A, 1190, 18–26.

    Article  CAS  Google Scholar 

  21. Perçin, I., Baydemir, G., Ergün, B., & Denizli, A. (2013). Artificial Cells, Nanomedicine, and Biotechnology, 41, 172–177.

    Article  Google Scholar 

  22. Baydemir, G., & Denizli, A. (2014). Artificial Cells, Nanomedicine, and Biotechnology. doi:10.3109/21691401.2014.897631.

    Google Scholar 

  23. Lozinsky, V. I., Galaev, I. Y., Plieva, F. M., Savina, I. N., Jungvid, H., & Mattiasson, B. (2003). Trends in Biotechnology, 21, 445–451.

    Article  CAS  Google Scholar 

  24. Kumar, A., & Srivastava, A. (2010). Nature Protocols, 5, 1737–1747.

    Article  CAS  Google Scholar 

  25. Bereli, N., Şener, G., Yavuz, H., & Denizli, A. (2011). Materials Science and Engineering: C, 31, 1078–1083.

    Article  CAS  Google Scholar 

  26. Andaç, M., Galaev, I. Y., & Denizli, A. (2012). Journal of Separation Science, 35, 1173–1182.

    Article  Google Scholar 

  27. Perçin, I., Yavuz, H., Aksöz, E., & Denizli, A. (2012). Biotechnology Progress, 28, 756–761.

    Article  Google Scholar 

  28. Çimen, D., & Denizli, A. (2012). Colloids and Surfaces. B, Biointerfaces, 93, 29–35.

    Article  Google Scholar 

  29. Tekiner, P., Perçin, I., Ergün, B., Yavuz, H., & Aksöz, E. (2012). Journal of Molecular Recognition, 25, 549–554.

    Article  CAS  Google Scholar 

  30. Üzek, R., Uzun, L., Şenel, S., & Denizli, A. (2013). Colloids and Surfaces. B, Biointerfaces, 102, 243–250.

    Article  Google Scholar 

  31. Arvidsson, P., Plieva, F. M., Savina, I. N., Lozinsky, V. I., Fexby, S., Bülow, L., Galaev, I. Y., & Mattiasson, B. (2002). Journal of Chromatography. A, 977, 27–38.

    Article  CAS  Google Scholar 

  32. Alkan, H., Bereli, N., Baysal, Z., & Denizli, A. (2009). Biochemical Engineering Journal, 45, 201–208.

    Article  CAS  Google Scholar 

  33. Yılmaz, F., Bereli, N., Yavuz, H., & Denizli, A. (2009). Biochemical Engineering Journal, 43, 272–279.

    Article  Google Scholar 

  34. Zhang, G., Cao, Q., Li, N., Li, K., & Liu, F. (2011). Talanta, 83, 1515–1520.

    Article  CAS  Google Scholar 

  35. Garcia-Diego, C., & Cuellar, J. (2008). Chemical Engineering Journal, 143, 337–348.

    Article  CAS  Google Scholar 

  36. del Pilar Ferraris, M., Barrera, G. I., Padilla, A. P., & Rodríguez, J. A. (2011). Journal of Chromatography B, 879, 2741–2745.

    Article  Google Scholar 

  37. Denizli, A., Yavuz, H., Garipcan, B., & Arıca, M. Y. (2000). Journal of Applied Polymer Science, 76, 115–124.

    Article  CAS  Google Scholar 

  38. Öncel, Ş., Uzun, L., Garipcan, B., & Denizli, A. (2005). Industrial and Engineering Chemistry Research, 44, 7049–7056.

    Article  Google Scholar 

  39. Denizli, F., Denizli, A., & Arica, M. Y. (1999). Polymer International, 48, 360–366.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Molecular Biology Division, Hacettepe University, Beytepe, 06800, Ankara, Turkey

    Işık Perçin

  2. Department of Chemistry, Biochemistry Division, Hacettepe University, Beytepe, 06800, Ankara, Turkey

    Gulsu Sener, Ahmet Hamdi Demirçelik, Nilay Bereli & Adil Denizli

  3. Institute of Science, Nanotechnology and Nanomedicine Division, Hacettepe University, Beytepe, 06800, Ankara, Turkey

    Gulsu Sener

Authors
  1. Işık Perçin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gulsu Sener
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ahmet Hamdi Demirçelik
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nilay Bereli
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Adil Denizli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adil Denizli.

Additional information

Cibacron Blue F3GA and Alkali Blue 6B immobilized poly(hydroxyethyl methacrylate) [PHEMA] cryogel discs were prepared.

Equilibrium lysozyme concentration, pH, ionic strength and temperature on lysozyme adsorption were investigated.

Purification of lysozyme from chicken egg white was performed by using Cibacron Blue F3GA and Alkali Blue 6B immobilized poly(hydroxyethyl methacrylate) [PHEMA] cryogel discs.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Perçin, I., Sener, G., Demirçelik, A.H. et al. Comparison of Two Different Reactive Dye Immobilized Poly(Hydroxyethyl Methacrylate) Cryogel Discs for Purification of Lysozyme. Appl Biochem Biotechnol 175, 2795–2805 (2015). https://doi.org/10.1007/s12010-014-1454-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-014-1454-5

Keywords

Search

Navigation