Abstract
In this study, citric acid was produced from waste cooking oil by Yarrowia lipolytica SWJ-1b. To get the maximal yield of citric acid, the compositions of the medium for citric acid production were optimized, and our results showed that extra nitrogen and magnesium rather than vitamin B1 and phosphate were needed for CA accumulation when using waste cooking oil. The results also indicated that the optimal initial concentration of the waste cooking oil in the medium for citric acid production was 80.0 g/l, and the ideal inoculation size was 1 × 107 cells/l of medium. We also reported that during 10-l fermentation, 31.7 g/l of citric acid, 6.5 g/l of isocitric acid, 5.9 g/l of biomass, and 42.1 g/100.0 g cell dry weight of lipid were attained from 80.0 g/l of waste cooking oil within 336 h. At the end of the fermentation, 94.6 % of the waste cooking oil was utilized by the cells of Y. lipolytica SWJ-1b, and the yield of citric acid was 0.4 g/g waste cooking oil, which suggested that waste cooking oil was a suitable carbon resource for citric acid production.
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This work was supported by Grant 31200023 from the National Natural Science Foundation of China and Grant BK20130416 from the Provincial Natural Science Foundation of Jiangsu.
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Liu, X., Lv, J., Xu, J. et al. Citric Acid Production in Yarrowia lipolytica SWJ-1b Yeast When Grown on Waste Cooking Oil. Appl Biochem Biotechnol 175, 2347–2356 (2015). https://doi.org/10.1007/s12010-014-1430-0
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DOI: https://doi.org/10.1007/s12010-014-1430-0