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A New GH43 α-Arabinofuranosidase from Humicola insolens Y1: Biochemical Characterization and Synergistic Action with a Xylanase on Xylan Degradation

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Abstract

A new α-arabinofuranosidase gene (Hiabf43) was cloned from Humicola insolens Y1 and successfully expressed in Pichia pastoris GS115. Deduced HiAbf43 contained a putative signal peptide and a catalytic domain of glycoside hydrolase (GH) family 43. Purified recombinant HiAbf43 showed optimal activity at pH 5.0 and 50 °C, and was active over a broad pH range. The enzyme was specific for the cleavage of α-1,3-linkage and showed high activity against 4-nitrophenyl α-l-arabinofuranoside, debranched arabinan, and sugar beet arabinan. Sequential addition of HiAbf43 followed by Xyn11A increased the degradation efficiency of birchwood and beechwood xylans but not wheat arabinoxylan. The synergy degree was high up to 1.21-fold.

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Acknowledgments

This research was supported by the National Science Foundation for Distinguished Young Scholars of China (31225026) and the National Natural Science Foundation of China (31172235) and the China Modern Agriculture Research System (CARS-42).

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Authors and Affiliations

  1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing, 100081, People’s Republic of China

    Xinzhuo Yang, Pengjun Shi, Rui Ma, Huiying Luo, Huoqing Huang, Peilong Yang & Bin Yao

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  1. Xinzhuo Yang
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  2. Pengjun Shi
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  3. Rui Ma
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  4. Huiying Luo
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  5. Huoqing Huang
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  6. Peilong Yang
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  7. Bin Yao
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Correspondence to Bin Yao.

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Yang, X., Shi, P., Ma, R. et al. A New GH43 α-Arabinofuranosidase from Humicola insolens Y1: Biochemical Characterization and Synergistic Action with a Xylanase on Xylan Degradation. Appl Biochem Biotechnol 175, 1960–1970 (2015). https://doi.org/10.1007/s12010-014-1416-y

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  • DOI: https://doi.org/10.1007/s12010-014-1416-y

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