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A Substrate-Based Approach for the Selection of Oil-Bearing Heterotrophs from Nitrogen-Deficient Soil for Lipid Production

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Abstract

In this study, nine heterotrophic yeast isolates were tested for their ability to assimilate crude glycerol and consecutive conversion to triacylglycerides (TGAs). All the organisms were initially screened on crude glycerol-based selection media, and those producing lipid globules were further evaluated for lipid production. Sudan Black B staining of eight isolates showed lipid globules. These strains were further studied at different C/N ratio. The molecular identification revealed that the isolates belonged to the genera of Yarrowia and Candida. Among these isolates, SKY7 (Yarrowia lipolytica) produced up to 42.04 ± 0.11 % of lipid w/w) with a C/N ratio of 100 and fermentation time of 72 h. The other strains produced 5.82 ± 0.4 to 34.57 ± 0.44 % lipid (w/w). The GC-flame ionization detector (FID) lipid profile showed that the lipid produced by the strains had close resemblance with vegetable oil and could serve as a feedstock for biodiesel production. Biolog test of the isolates revealed a wide spectrum of carbon utilization.

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Acknowledgments

Authors would like to acknowledge the Natural Sciences and Engineering Research Council of Canada (grant A4984, strategic grant 412994-11, Canada Research Chairs) for financial support. We are grateful to technical staffs of INRS-ETE, for their timely help to analyze the samples on GC-FID.

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Authors and Affiliations

  1. Institut national de la recherche scientifique, Centre Eau, Terre & Environnement, Université du Québec, 490, rue de la Couronne, Quebec, G1K 9A9, Canada

    M. Kuttiraja, Shyam Krishna, Ayed Dhouha & Rajeshwar Dayal Tyagi

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  1. M. Kuttiraja
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  2. Shyam Krishna
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  3. Ayed Dhouha
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  4. Rajeshwar Dayal Tyagi
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Correspondence to Rajeshwar Dayal Tyagi.

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Kuttiraja, M., Krishna, S., Dhouha, A. et al. A Substrate-Based Approach for the Selection of Oil-Bearing Heterotrophs from Nitrogen-Deficient Soil for Lipid Production. Appl Biochem Biotechnol 175, 1926–1937 (2015). https://doi.org/10.1007/s12010-014-1378-0

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  • DOI: https://doi.org/10.1007/s12010-014-1378-0

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