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Production and Characterization of β-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31

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Abstract

In this paper, several agro-industrial wastes (soybean meal and wheat straw, rice and peanut husks, corn cob and corn stover, and sugarcane bagasse) were tested for the production of β-glucosidase by the cultivation of thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state fermentation (SSF). Among the tested substrates, the highest yields were obtained in soybean meal. Other fermentation parameters were also evaluated, such as initial pH, merge substrates, and fermentation time, as well as the physicochemical characterization of the enzyme. The best results were obtained after 192 h of fermentation with the initial pH adjusted to 6.0. The substrate mixture did not improve the enzyme production by microorganism. The β-glucosidase showed best catalytic activity at pH 4.5 and at 75 °C and remained stable in the pH range from 4.5 to 9.5 and the temperature range 40–75 °C. The enzyme showed 80 % of its activity at a concentration of 15 mM glucose and remained stable up to 20 % ethanol.

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Acknowledgments

The authors wish to thank Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Pesquisa (CNPq) for financial support.

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Authors and Affiliations

  1. Laboratory of Biochemistry and Applied Microbiology, Universidade Estadual Paulista (UNESP), Av. Cristóvão Colombo, 2265, 15054-000, São José do Rio Preto, São Paulo, Brazil

    Josiani de Cassia Pereira, Heloiza Ferreira Alves do Prado, Daniela Alonso Bocchini Martins, Eleni Gomes & Roberto da Silva

  2. Biological na Environment Sciences Faculty, FCBA, Universidade Federal da Grande Dourados (UFGD), Km 12, Rodovia Dourados—Itahum, Dourados, MS, 79804-970, Brazil

    Rodrigo Simões Ribeiro Leite

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  1. Josiani de Cassia Pereira
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  2. Rodrigo Simões Ribeiro Leite
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  3. Heloiza Ferreira Alves do Prado
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  4. Daniela Alonso Bocchini Martins
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  5. Eleni Gomes
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  6. Roberto da Silva
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Correspondence to Josiani de Cassia Pereira.

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de Cassia Pereira, J., Leite, R.S.R., do Prado, H.F.A. et al. Production and Characterization of β-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31. Appl Biochem Biotechnol 175, 723–732 (2015). https://doi.org/10.1007/s12010-014-1332-1

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  • DOI: https://doi.org/10.1007/s12010-014-1332-1

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