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Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor

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Abstract

Lycopene biosynthesis by Blakeslea trispora was greatly enhanced in a stirred-tank reactor when a nonsynchronous inoculation process, in which the (+) mating type was inoculated after the (−) mating type has been grown for a certain period of time, was applied. The lycopene concentration with nonsynchronous inoculation in a 24-h inoculation interval was 33 % higher than that with synchronous inoculation. The optimum inoculation ratio was 1:2 (+/−) at the 36 and 48 h inoculum age of mating types (+) and (−), respectively. Fermentation time for the individual strains and mated conditions showed that the (+) mating type grows faster than the (−) mating type. Morphological observation showed that the mycelium ratio of B. trispora (−) in mating culture with nonsynchronous inoculation was higher than that with synchronous inoculation. The results indicated that nonsynchronous inoculation process increased the dominance of B. trispora (−) in joint cultivation and hence stimulated lycopene biosynthesis.

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References

  1. Rao, A. V., & Agarwal, S. (2000). Journal of the American College of Nutrition, 19(5), 563–569.

    Article  CAS  Google Scholar 

  2. Rao, A., & Agarwal, S. (1999). Nutrition Research, 19(2), 305–323.

    Article  CAS  Google Scholar 

  3. Kong, K.-W., Khoo, H.-E., Prasad, K. N., Ismail, A., Tan, C.-P., & Rajab, N. F. (2010). Molecules, 15(2), 959–987.

    Article  CAS  Google Scholar 

  4. Swarthout, E. J. (1963), U.S. Pat. No. 3,097,146, Publication date: 09-07-1963.

  5. Cerdá-Olmedo, E. (1989), Production of carotenoids with fungi. In biotechnology of vitamins, pigments and growth factors. Springer, pp 27–42.

  6. Ye, Z. W., Jiang, J. G., & Wu, G. H. (2008). Biotechnology Advances, 26(4), 352–360.

    Article  CAS  Google Scholar 

  7. Aksu, Z., & Eren, A. T. (2005). Process Biochemistry, 40(9), 2985–2991.

    Article  CAS  Google Scholar 

  8. Kim, S.-W., Kim, J.-B., Ryu, J.-M., Jung, J.-K., & Kim, J.-H. (2009). Process Biochemistry, 44(8), 899–905.

    Article  CAS  Google Scholar 

  9. Pegklidou, K., Mantzouridou, F., & Tsimidou, M. Z. (2008). Journal of Agricultural and Food Chemistry, 56(12), 4482–4490.

    Article  CAS  Google Scholar 

  10. Mantzouridou, F., & Tsimidou, M. Z. (2008). Trends in Food Science and Technology, 19(7), 363–371.

    Article  CAS  Google Scholar 

  11. Lopez-Nieto, M. J., Costa, J., Peiro, E., Mendez, E., Rodriguez-Saiz, M., de la Fuente, J. L., Cabri, W., & Barredo, J. L. (2004). Applied Microbiology and Biotechnology, 66(2), 153–159.

    Article  CAS  Google Scholar 

  12. Vereschagina, O. A., Memorskaya, A. S., & Tereshina, V. M. (2010). Microbiology, 79(5), 593–601.

    Article  CAS  Google Scholar 

  13. Goksungur, Y., Mantzouridou, F., Roukas, T., & Kotzekidou, P. (2004). Applied Biochemistry and Biotechnology, 112(1), 37–54.

    Article  CAS  Google Scholar 

  14. Varzakakou, M., & Roukas, T. (2009). Preparative Biochemistry and Biotechnology, 40(1), 76–82.

    Article  Google Scholar 

  15. Mantzouridou, F., Naziri, E., & Tsimidou, M. Z. (2008). Journal of Agricultural and Food Chemistry, 56(8), 2668–2675.

    Article  CAS  Google Scholar 

  16. Vereshchagina, O., Memorskaya, A., Kochkina, G., & Tereshina, V. (2012). Microbiology, 81(5), 517–525.

    Article  CAS  Google Scholar 

  17. Thomas, D., & Goodwin, T. (1967). Phytochemistry, 6(3), 355–360.

    Article  CAS  Google Scholar 

  18. Van den Ende, H. (1968). Journal of Bacteriology, 96(4), 1298–1303.

    Google Scholar 

  19. Schmidt, A. D., Heinekamp, T., Matuschek, M., Liebmann, B., Bollschweiler, C., & Brakhage, A. A. (2005). Applied Microbiology and Biotechnology, 67(4), 549–555.

    Article  CAS  Google Scholar 

  20. Sun, Y., Yuan, Q.-P., & Vriesekoop, F. (2007). Process Biochemistry, 42(10), 1460–1464.

    Article  CAS  Google Scholar 

  21. Bohme, K., Richter, C., & Patz, R. (2006). Biotechnology Journal, 1(10), 1080–1084.

    Article  Google Scholar 

  22. Varzakakou, M., Roukas, T., & Kotzekidou, P. (2010). World Journal of Microbiology and Biotechnology, 26(12), 2151–2156.

    Article  CAS  Google Scholar 

  23. Nanou, K., & Roukas, T. (2011). Bioresource Technology, 102(17), 8159–8164.

    Article  CAS  Google Scholar 

  24. Nanou, K., Roukas, T., & Papadakis, E. (2011). Biochemical Engineering Journal, 54(3), 172–177.

    Article  CAS  Google Scholar 

  25. Wang, Q., Luo, W., Gu, Q.-Y., Feng, L.-R., Li, H.-G., & Yu, X.-B. (2013). Applied Biochemistry and Biotechnology, 171(7), 1692–1700.

    Article  CAS  Google Scholar 

  26. Shi, Y. Q., Xin, X. L., & Yuan, Q. P. (2012). Biotechnology Letters, 34(5), 849–852.

    Article  CAS  Google Scholar 

  27. Miller, G. L. (1959). Analytical Chemistry, 31(3), 426–428.

    Article  CAS  Google Scholar 

  28. Jüsten, P., Paul, G., Nienow, A., & Thomas, C. (1996). Biotechnology and Bioengineering, 52(6), 672–684.

    Article  Google Scholar 

  29. Ciegler, A., Arnold, M., & Anderson, R. (1959). Applied Microbiology, 7(2), 94–98.

    CAS  Google Scholar 

  30. Mantzouridou, F., & Tsimidou, M. Z. (2007). European Journal of Lipid Science and Technology, 109(1), 3–10.

    Article  CAS  Google Scholar 

  31. Mantzouridou, F., Tsimidou, M. Z., & Roukas, T. (2006). Journal of Agricultural and Food Chemistry, 54(7), 2575–2581.

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions “the 111 Project” (Grant No. 111-2-06) and the Natural Science Foundation of Jiangsu Province, China (Grant No. BK20130130).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Qiang Wang, Ling-ran Feng, Wei Luo, Han-guang Li, Ya Zhou & Xiao-bin Yu

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  1. Qiang Wang
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  2. Ling-ran Feng
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  3. Wei Luo
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  4. Han-guang Li
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  5. Ya Zhou
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  6. Xiao-bin Yu
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Correspondence to Xiao-bin Yu.

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Wang, Q., Feng, Lr., Luo, W. et al. Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor. Appl Biochem Biotechnol 175, 770–779 (2015). https://doi.org/10.1007/s12010-014-1327-y

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  • DOI: https://doi.org/10.1007/s12010-014-1327-y

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