Skip to main content
SpringerLink
Log in

Opposing Effects of External Gibberellin and Daminozide on Stevia Growth and Metabolites

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Steviol glycosides (SVglys) and gibberellins are originated from the shared biosynthesis pathway in Stevia (Stevia rebaudiana Bertoni). In this research, two experiments were conducted to study the opposing effects of external gibberellin (GA3) and Daminozide (a gibberellin inhibitor) on Stevia growth and metabolites. Results showed that GA3 significantly increased the stem length and stem dry weight in Stevia. Total soluble sugar content increased while the SVglys biosynthesis was decreased by external GA3 applying in Stevia leaves. In another experiment, the stem length was reduced by Daminozide spraying on Stevia shoots. The Daminozide did not affect the total SVglys content, while in 30 ppm concentration, significantly increased the soluble sugar production in Stevia leaves. Although the gibberellins biosynthesis pathway has previously invigorated in Stevia leaf, the Stevia response to external gibberellins implying on high precision regulation of gibberellins biosynthesis in Stevia and announces that Stevia is able to kept endogenous gibberellins in a low quantity away from SVglys production. Moreover, the assumption that the internal gibberellins were destroyed by Daminozide, lack of Daminozide effects on SVglys production suggests that gibberellins biosynthesis could not act as a competitive factor for SVglys production in Stevia leaves.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

SVglys:

Steviol glycosides

GA:

Gibberellic acid

DAM:

Daminozide

Stev:

Stevioside

Reb A:

Rebaudioside A

Reb F:

Rebaudioside F

Reb C:

Rebaudioside C

Dulc A:

Dulcoside A

HI:

Harvest index

TSS:

Total soluble sugar

References

  1. Yadav, A. K., Singh, S., Dhyani, D., & Ahuja, P. S. (2011). Canadian Journal of Plant Science, 91, 1–27.

    Article  Google Scholar 

  2. Gregersen, S., Jeppesen, P. B., Holst, J. J., & Hermansen, K. (2004). Metabolism, 53, 73–76.

    Article  CAS  Google Scholar 

  3. Brandle, J., & Telmer, P. (2007). Phytochemistry, 68, 1855–1863.

    Article  CAS  Google Scholar 

  4. Sharma, M., Thakral, N. K., & Thakral, S. (2009). Natural Product Radiance, 8, 181–189.

    Google Scholar 

  5. Bondarev, N., Sukhanova, M., Reshetnyak, O., & Nosov, A. (2003). Biologia Plantarum, 47, 261–264.

    Article  CAS  Google Scholar 

  6. Starratt, A. N., Kirby, C. W., Pocs, R., & Brandle, J. E. (2002). Phytochemistry, 59, 367–370.

    Article  CAS  Google Scholar 

  7. Kim, K. K., Sawa, Y., & Shibata, H. (1996). Archives of Biochemistry and Biophysics, 332, 223–230.

    Article  CAS  Google Scholar 

  8. Hedden, P., & Kamiya, Y. (1997). Annual Review of Plant Physiology and Plant Molecular Biology, 48, 431–460.

    Article  CAS  Google Scholar 

  9. Kamiya, Y., Nakayama, I., Kobayashi, M. (1992). In Progress in plant growth regulation (pp. 555–565), Springer.

  10. Lange, T., Kappler, J., Fischer, A., Frisse, A., Padeffke, T., Schmidtke, S., & Lange, M. J. P. (2005). Plant Physiology, 139, 213–223.

    Article  CAS  Google Scholar 

  11. Sakamoto, T., Miura, K., Itoh, H., Tatsumi, T., Ueguchi-Tanaka, M., Ishiyama, K., Kobayashi, M., Agrawal, G. K., Takeda, S., & Abe, K. (2004). Plant Physiology, 134, 1642–1653.

    Article  CAS  Google Scholar 

  12. Shibata, H., Sawa, Y., Oka, T.-A., Sonoke, S., Kim, K. K., & Yoshioka, M. (1995). Archives of Biochemistry and Biophysics, 321, 390–396.

    Article  CAS  Google Scholar 

  13. Rademacher, W. (2000). Annual Review of Plant Biology, 51, 501–531.

    Article  CAS  Google Scholar 

  14. Azuma, T., Ueno, S., Uchida, N., & Yasuda, T. (1997). Physiologia Plantarum, 99, 517–522.

    Article  CAS  Google Scholar 

  15. Dai, Z., Pang, Y., Xiao, Y., Qin, W., Yao, Y., Zhang, Z., & Pan, Y. (2010). Acta Agriculturae Shanghai, 26, 69–71.

    Google Scholar 

  16. Rademacher, W. (1992). On the mode of action of acylcylcohexanediones—a new type of plant growth retardant with possible relationships to daminozide. VII International Symposium on Plant Growth Regulators in Fruit Production, 329, 31–34.

    Google Scholar 

  17. Schmiderer, C., Grausgruber-Gröger, S., Grassi, P., Steinborn, R., & Novak, J. (2010). Journal of Plant Physiology, 167, 779–786.

    Article  CAS  Google Scholar 

  18. Roepke, J., Jean, T., Perkel, K. J., Blom, T., & Bozzo, G. G. (2013). Journal of Plant Growth Regulation, 32, 453–460.

    Article  CAS  Google Scholar 

  19. Barbara, K., Ewa, K., Jerzy, K., & Aleksander, C. (2006). Plant Growth Regulation, 48, 13–19.

    Article  CAS  Google Scholar 

  20. Ceunen, S., Geuns, J. (2013). Plant Science, 198, 72-82.

  21. Karimi, M., Ahmadi, A., Hashemi, J., Abbasi, A., & Angelini, L. G. (2014). Acta Physiologiae Plantarum, 36, 1211–1219.

    Article  Google Scholar 

  22. Geuns, J., Struyf, T. (2009). EUSTAS Round-Robin Testing of Steviol Glycosides. Proceedings of the 3rd EUSTAS Stevia Symposium, pp. 35–48.

  23. Geuns, J. M. (2010). Euprint  ed.  2010:307. Parkbosstraat 3, 3001 Heverlee, Belgium.

  24. Tobias, R. B., Boyer, C. D., & Shannon, J. C. (1992). Plant Physiology, 99, 146–152.

    Article  CAS  Google Scholar 

  25. Guglielminetti, L., Perata, P., & Alpi, A. (1995). Plant Physiology, 108, 735–741.

    CAS  Google Scholar 

  26. Alves, L. M., & Ruddat, M. (1979). Plant and Cell Physiology, 20, 123–130.

    CAS  Google Scholar 

  27. Mansouri, H., Asrar, Z., & Amarowicz, R. (2011). Acta Physiologiae Plantarum, 33, 1085–1091.

    Article  CAS  Google Scholar 

  28. Loreti, E., Povero, G., Novi, G., Solfanelli, C., Alpi, A., & Perata, P. (2008). New Phytologist, 179, 1004–1016.

    Article  CAS  Google Scholar 

  29. Cheikh, N., Brenner, M. L., Huber, J. L., & Huber, S. C. (1992). Plant Physiology, 100, 1238–1242.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    Mojtaba Karimi, Ali Ahmadi & Alireza Abbasi

  2. Department of Secondary Metabolites, Branch of Central Region of Iran, Agricultural Biotechnology Research Institute of Iran, (ABRII), Karaj, Iran

    Javad Hashemi

  3. Laboratory of Plant Physiology, Center of Agricultural Sciences, Federal University of Alagoas, Maceió, Brazil

    Antonio Pompeiano

  4. Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy

    Silvia Tavarini, Lorenzo Guglielminetti & Luciana G. Angelini

Authors
  1. Mojtaba Karimi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Javad Hashemi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ali Ahmadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alireza Abbasi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Antonio Pompeiano
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Silvia Tavarini
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lorenzo Guglielminetti
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Luciana G. Angelini
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mojtaba Karimi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Karimi, M., Hashemi, J., Ahmadi, A. et al. Opposing Effects of External Gibberellin and Daminozide on Stevia Growth and Metabolites. Appl Biochem Biotechnol 175, 780–791 (2015). https://doi.org/10.1007/s12010-014-1310-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-014-1310-7

Keywords

Search

Navigation