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Inhibitory Effects of Extract from G. lanceolata on LPS-Induced Production of Nitric Oxide and IL-1β via Down-regulation of MAPK in Macrophages

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Abstracts

Grateloupia lanceolata is a red alga native to coastal areas of East Asia. In this study, extract from G. lanceolata (EGL) was investigated for suppressive effects on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. EGL was found to have anti-inflammatory properties with the inhibition of nitric oxide (NO), pro-inflammatory cytokine production, and MAPK signaling in LPS-induced RAW 264.7 macrophages. Moreover, treatment of RAW 264.7 macrophage with EGL inhibited LPS-induced IL-1β production in a dose-dependent manner. These inhibitory effects were found with the blockage of p38 mitogen-activated protein kinases (MAPK), extracellular signal regulated kinases 1 and 2 (ERK1/2), and also c-Jun N-terminal kinases 1 and 2 (JNK1/2). These results indicated that anti-inflammatory actions of EGL in RAW 264.7 macrophages involved in the inhibition of LPS-induced p38MAPK/ERK/JNK signaling pathways. In addition, our findings suggest that EGL holds great promise for use in the treatment of various inflammatory diseases.

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The authors have no financial conflict of interest.

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Authors and Affiliations

  1. Department of Biology, Immunology Research Laboratory, BK21-Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Dong-gu, Gwangju, 501-759, Republic of Korea

    Dong Hwan Kim, Mi Eun Kim & Jun Sik Lee

  2. Recobio Company, Business Incubator Center, Chosun University, Dong-gu, Gwangju, 501-759, Republic of Korea

    Mi Eun Kim & Jun Sik Lee

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  1. Dong Hwan Kim
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  2. Mi Eun Kim
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Correspondence to Jun Sik Lee.

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Kim, D.H., Kim, M.E. & Lee, J.S. Inhibitory Effects of Extract from G. lanceolata on LPS-Induced Production of Nitric Oxide and IL-1β via Down-regulation of MAPK in Macrophages. Appl Biochem Biotechnol 175, 657–665 (2015). https://doi.org/10.1007/s12010-014-1301-8

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  • DOI: https://doi.org/10.1007/s12010-014-1301-8

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