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The Effects of Metal Ions in Euphorbia cf. lactea Latex on the Fibrinogenolytic Activity of a Plant Protease

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Abstract

Two synchrotron-based techniques, synchrotron X-ray fluorescence (SXRF) and X-ray absorption spectroscopy (XAS), have demonstrated that Ca2+ and Zn2+ were the major metal ions distributed in the natural latex of Euphorbia cf. lactea. Both metal ions were found to affect the fibrinogenolytic activity of a homodimeric protease purified from the latex of this plant. The dimeric protein had an estimated molecular mass of about 82 kDa analyzed by SDS-PAGE. Therefore this protein was called as EuP-82. Based on the results of circular dichroism (CD) spectroscopy and the fibrinolytic activity measurement, it was found that Ca2+ could activate the proteolytic activity of the enzyme by stabilizing its backbone structure. The intact conformation of EuP-82 was predicted from CD spectrum, which consisted of 51 % α-helix and 9 % β-sheet. Zn2+ (10 mM) could decrease the fibrinolytic activity of EuP-82 to 30 ± 1 %. CD spectrum also supported that the inhibitory effect of Zn2+ on the enzyme activity occurred by the drastic change of the enzyme structure with increasing the random coil conformation and by switching between α-helix and β-sheet structure. These results could be of first importance for further application to use EuP-82, the natural source protease as a potential drug for the thrombosis treatment. The fibrinolytic activity of EuP-82 may be enhanced by plasma Ca2+ which generally involves in human hemostasis system.

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Acknowledgments

This work was supported by the Suranaree University of Technology Research Fund for promoting academic accomplishment to be published in international journal. We also thank the Synchrotron Light Research Institute (public organization) for giving the facility to perform SXRF and XAS experiments at BL5.2: SUT-NANOTEC-SLRI Beamline.

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Authors and Affiliations

  1. School of Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand

    Jaruwan Siritapetawee

  2. Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, Thailand

    Wanwisa Limphirat & Chanapa Kongmark

  3. Department of Biological Sciences, Faculty of Sciences, Ubon Ratchathani University, Ubon Ratchathani, Thailand

    Chortip Kantachot

  4. Department of Materials Science, Faculty of Science, Kasetsart University, Bangkok, Thailand

    Chanapa Kongmark

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  1. Jaruwan Siritapetawee
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  2. Wanwisa Limphirat
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  3. Chortip Kantachot
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  4. Chanapa Kongmark
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Correspondence to Jaruwan Siritapetawee.

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Siritapetawee, J., Limphirat, W., Kantachot, C. et al. The Effects of Metal Ions in Euphorbia cf. lactea Latex on the Fibrinogenolytic Activity of a Plant Protease. Appl Biochem Biotechnol 175, 232–242 (2015). https://doi.org/10.1007/s12010-014-1255-x

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  • DOI: https://doi.org/10.1007/s12010-014-1255-x

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