Abstract
Alkali/acid-pretreated canola meal and mustard bran were subjected to endo-1,4-β-xylanase (T. longibrachiatum) hydrolysis for oligosaccharide production. Pretreatments significantly (α = 0.05) increased the relative content of pentose sugars, especially in alkali-pretreated canola meal (∼44 %) and mustard bran (∼72 %). The amounts of pentosan (g/100 g) in acid- and alkali-pretreated canola meal were 7.50 and 8.21 and in corresponding mustard bran were 8.67 and 10.39, respectively. These pretreated substrates produced a pentose content (g/100 g) of 2.10 ± 0.14 (18 h) and 2.95 ± 0.10 (24 h), respectively, during hydrolysis. As per UPLC-MS data, the main oligosaccharides in the hydrolyzates of alkali-pretreated substrates are xylo-glucuronic acid and xylobiose. The release of total phenolics of the hydrolyzates increased until 18 h irrespective of the type of substrate or pretreatment. Hydrolyzates of acid-pretreated substrates indicated more total antioxidant activity than alkali-pretreated substrates, attributed to its high phenolic content. The study suggests the potential of canola meal and mustard bran for the production of oligosaccharides, wherein the use of various combinations of cell-wall-degrading enzymes and its optimization may result in a better yield, with simultaneous production of endogenous phenolics.
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Acknowledgments
This study was funded by the Agri-Food Research and Development Initiative (ARDI)-Growing Forward 1 Program and supported by the Bunge Canada, Canola Council of Canada and GS Dunn Ltd. The authors would like to thank Dr. Steve Cui and his technician Ms. Cathy Wang from the Guelph Food Research Centre, Agriculture and Agri-Food Canada for carrying out the neutral sugar analysis. The authors also would like to thank Hai Feng, Department of Human Nutritional Sciences, University of Manitoba (Winnipeg, MA, Canada) for technical assistance with UPLC-MS.
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Yuan, L., Scanlon, M.G., Eskin, N.A.M. et al. Effect of Pretreatments and Endo-1,4-β-Xylanase Hydrolysis of Canola Meal and Mustard Branfor Production of Oligosaccharides. Appl Biochem Biotechnol 175, 194–208 (2015). https://doi.org/10.1007/s12010-014-1253-z
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DOI: https://doi.org/10.1007/s12010-014-1253-z