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In Vitro Callus Culture of Heliotropium indicum Linn. for Assessment of Total Phenolic and Flavonoid Content and Antioxidant Activity

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Abstract

The total phenolic and flavonoid content and percentage of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of callus and in vivo plant parts of Heliotropium indicum Linn. were estimated. Murashige and Skoog (MS) basal medium supplemented with α-naphthaleneacetic acid (NAA) 2.0 mg/l with benzyladenine (BA) 0.5 mg/l showed the highest amount of callus biomass (1.87 g/tube). The morphology of callus was significantly different according to the plant growth regulators and their concentrations used in the medium. The highest amount of total phenolic (21.70 mg gallic acid equivalent per gram (GAE/g)) and flavonoid (4.90 mg quercetin equivalent per gram (QE/g)) content and the maximum percentage (77.78 %) of radical scavenging activity were estimated in the extract of inflorescence. The synergistic effect of NAA (2.0 mg/l) and BA (0.5 mg/l) enhances the synthesis of total phenolic (9.20 mg GAE/g) and flavonoid (1.25 mg QE/g) content in the callus tissue. The callus produced by the same concentration shows 45.24 % of free radical scavenging activity. While comparing the various concentrations of NAA with 2,4-dichlorophenoxyacetic acid (2,4-D) for the production of callus biomass, total phenolic and flavonoid content and free radical scavenging activity, all the concentrations of NAA were found to be superior than those of 2,4-D.

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References

  1. Oliveira, O. C., Valentim, I. B., Silva, C. A., Bechara, E. J. H., Barros, M. P., Mano, C. M., & Goulart, M. O. F. G. (2009). Food Chemistry, 115, 469–475.

    Article  Google Scholar 

  2. Prasad, K. N., Yang, B., Yang, S., Chen, Y., Zhao, M., Ashraf, M., & Jiang, Y. (2009). Food Chemistry, 116, 1–7.

    Article  Google Scholar 

  3. Matkowski, A. (2008). Biotechnology Advances, 26, 548–560.

    Article  CAS  Google Scholar 

  4. Miguel, M. G. (2010). Flavour and Fragrance Journal, 25(5), 291–312.

    Article  CAS  Google Scholar 

  5. Ali, S. S., Kasoju, N., Luthra, A., Singh, A., Sharanabasava, H., Sahu, A., & Bora, U. (2008). Food Research International, 41, 1–15.

    Article  Google Scholar 

  6. Vijaya, S. N., Udayasri, P. V., Aswani, K. Y., Ravi, B. B., Phani, K. Y., & Vijay, V. M. (2010). Journal of Natural Products, 3, 112–23.

    Google Scholar 

  7. Wong, K. F., & Taha, R. M. (2012). Research Journal of Biotechnology, 7(3), 75–80.

    CAS  Google Scholar 

  8. Lubaina, A. S., & Murugan, K. (2012). International Journal of Pharmacy and Pharmaceutical Sciences, 4(1), 334–336.

    CAS  Google Scholar 

  9. El-Baz, F. K., Mohamed, A. A., & Ali, S. I. (2010). Nova Biotechnologica, 10(2), 79–94.

    Google Scholar 

  10. Szopa, A., & Ekiert, H. (2012). Applied Biochemistry and Biotechnology, 166(8), 1941–1948.

    Article  CAS  Google Scholar 

  11. North, J. J., Ndakidemi, P. A., & Laubscher, C. P. (2012). International Journal of Physical Science, 7(4), 638–646.

    Article  CAS  Google Scholar 

  12. Anonymous. (1952). The Wealth of India: A Dictionary of Indian Raw Materials and Industrial Products. Publication and Information Directorate, CSIR, New Delhi, vol. V. pp. 9-30.

  13. Kugelman, M., Liu, W. C., Axelrod, M., Mc Bride, T. J., & Rao, K. V. (1976). Lloydia, 39, 125–128.

    CAS  Google Scholar 

  14. Meher, A. (2011). Internatinal Journal of Pharmaceutical Research and Development, 3(10), 1–4.

    Google Scholar 

  15. Dash, G. K., & Murthy, P. N. (2011). ISRN (International Scholarly Research Network) Pharmacology. doi:10.5402/2011/847980.

    Google Scholar 

  16. Ashoka, M. S., & Shashidhar, C. S. (2011). International Journal of Research in Ayurveda and Pharmacy, 2(3), 999–1002.

    Google Scholar 

  17. Adelaja, A. A., Ayoola, M. D., Otulana, J. O., Akinola, O. B., Olayiwola, A., & Ejiwunmi, A. B. (2008). Malaysian Journal of Medical Sciences, 15(3), 22–30.

    Google Scholar 

  18. Koffuor, G. A., Boye, A., Ameyaw, E. O., Amoateng, P., & Abaitey, A. K. (2012). International Research Journal of Pharmacy and Pharmacology, 2(5), 103–109.

    Google Scholar 

  19. Murashige, T., & Skoog, F. (1962). Physiologia Plantarum, 15, 473–497.

    Article  CAS  Google Scholar 

  20. Makkar, H. P. S., Becker, K., & Abel, H. (1997). Journal of the Science of Food and Agriculture, 75, 511–520.

    Article  CAS  Google Scholar 

  21. Chang, C., Yang, M., Wen, H., & Chem, J. (2002). Journal of Food and Drug Analysis, 1(10), 178–182.

    Google Scholar 

  22. Cuendet, M., Hostettmann, K., & Potterat, O. (1997). Helvetica ChimicaAct, 80, 1144–1152.

    Article  CAS  Google Scholar 

  23. Burits, M., & Bucar, F. (2000). Phytotherapy Research, 14, 323–328.

    Article  CAS  Google Scholar 

  24. Hamideh, J., Khosro, P., & Javad, N. D. M. (2012). Journal of Medicinal Plants Research, 6(18), 3407–3414.

    CAS  Google Scholar 

  25. Hazeena, M. S., & Sulekha, G. R. (2008). Journal of Tropical Agriculture, 46, 79–84.

    CAS  Google Scholar 

  26. Jin, X., Hu, X., Sun, Y., Zhang, D., & He, P. (2012). Hort Science, 47(6), 790–792.

    CAS  Google Scholar 

  27. Fatima, Z., Mujib, A., Fatima, S., Arshi, A., & Umar, S. (2009). Turkish Journal of Botany, 33, 393–405.

    Google Scholar 

  28. Bidchol, A. M., Wilfred, A., Abhijna, P., & Harish, R. (2011). Food and Bioprocess Technology, 4, 1137–1143.

    Article  Google Scholar 

  29. Radfar, M., Sudarshana, M. S., & Niranjan, M. H. (2012). Journal of Medicinal Plants Research, 6(12), 2443–2447.

    Article  CAS  Google Scholar 

  30. Bathoju, G., & Giri, A. (2012). Journal of Pharmacognosy, 3(2), 101–103.

    Google Scholar 

  31. Maneechai, S., De-Eknamkul, W., Umehara, K., Noguchi, H., & Likhitwitayawuid, K. (2012). Phytochemistry, 81, 42–9.

    Article  CAS  Google Scholar 

  32. Nikolaeva, T. N., Zagoskina, N. V., & Zaprometov, M. N. (2009). Russian Journal of Plant Physiology, 56, 45–49.

    Article  CAS  Google Scholar 

  33. Amid, A., Johan, N. N., Jamal, P., & Zain, W. N. W. M. (2011). African Journal of Biotechnology, 10, 18653–18656.

    CAS  Google Scholar 

Download references

Acknowledgments

One of the authors, Dr. M. Senthil Kumar, is thankful to University Grants Commission, New Delhi, India, for receiving financial support as a project fellow through a Major Research Project [F. No. 37-480/ 2009 (SR)] during the research work.

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The authors declare that they have no conflict of interest.

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Correspondence to Srinivasan Balachandran.

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Kumar, M.S., Chaudhury, S. & Balachandran, S. In Vitro Callus Culture of Heliotropium indicum Linn. for Assessment of Total Phenolic and Flavonoid Content and Antioxidant Activity . Appl Biochem Biotechnol 174, 2897–2909 (2014). https://doi.org/10.1007/s12010-014-1235-1

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  • DOI: https://doi.org/10.1007/s12010-014-1235-1

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