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A Yeast Isolated from Cashew Apple Juice and Its Ability to Produce First- and Second-Generation Ethanol

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Abstract

The aim of this study was to isolate and identify an indigenous yeast from cashew apple juice (CAJ) and then use it in the production of first- and second-generation ethanol, using CAJ and the enzymatic hydrolysate of cashew apple bagasse (MCAB-OH), respectively. The isolated yeast was identified as belonging to the genus Hanseniaspora. Afterward, the effect of the medium initial pH on the production of ethanol from CAJ was evaluated in the range of 3.0 to 5.5, with its maximum ethanol production of 42 g L−1 and Y P/S of 0.44 g g−1 and 96 % efficiency. The effect of temperature (28–38 °C) on ethanol production was evaluated in a synthetic medium, and no difference in ethanol production in the temperature range evaluated (28–36 °C) was observed. At 32 °C, the yield, concentration, efficiency, and productivity of ethanol when using the CAJ medium were higher when compared to the results achieved for the synthetic medium. Regarding second-generation ethanol, the results showed that the yeast produced 24.37 g L−1 of ethanol with an efficiency of 80.23 % and a productivity of 4.87 g L−1 h−1 at 5 h. Therefore, Hanseniaspora sp., isolated from CAJ, is a promising microorganism for the production of first- and second-generation ethanol.

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Abbreviations

CAJ:

Cashew apple juice

CAB:

Cashew apple bagasse

MCAB-OH:

Enzymatic hydrolysate of cashew apple bagasse

P max :

Maximum ethanol concentration (g L−1)

NS:

non-Saccharomyces yeasts

η :

Efficiency of sugar conversion to ethanol (%)

Q P :

Ethanol volumetric productivity (g L h−1)

θ S :

Substrate conversion (%)

Y P/X :

Yield of product based on cell growth (g g−1)

Y X/S :

Yield of cell growth based on substrate consumption (g g−1)

Y P/S :

Yield of product based on substrate consumption (g g−1)

μ max :

Maximum specific growth rate of cells (h−1)

FPU:

Filter paper unit

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Acknowledgments

The authors would like to thank the Brazilian research-funding agencies CNPq and CAPES.

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Authors and Affiliations

  1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, 604440-554, Fortaleza, CE, Brazil

    E. M. Barros, T. H. S. Rodrigues, A. D. T. Pinheiro, M. V. P. Rocha & Luciana R. B. Gonçalves

  2. Departamento de Biologia, Universidade Federal do Ceará, Campus do Pici, Bloco 909, 60455-760, Fortaleza, CE, Brazil

    A. L. Angelim & V. M. M. Melo

Authors
  1. E. M. Barros
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  2. T. H. S. Rodrigues
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  3. A. D. T. Pinheiro
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  4. A. L. Angelim
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  5. V. M. M. Melo
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  6. M. V. P. Rocha
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  7. Luciana R. B. Gonçalves
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Correspondence to Luciana R. B. Gonçalves.

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Barros, E.M., Rodrigues, T.H.S., Pinheiro, A.D.T. et al. A Yeast Isolated from Cashew Apple Juice and Its Ability to Produce First- and Second-Generation Ethanol. Appl Biochem Biotechnol 174, 2762–2776 (2014). https://doi.org/10.1007/s12010-014-1224-4

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  • DOI: https://doi.org/10.1007/s12010-014-1224-4

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