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Expressed Proteins of Herbaspirillum seropedicae in Maize (DKB240) Roots-Bacteria Interaction Revealed Using Proteomics

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Abstract

Several molecular tools have been used to clarify the basis of plant-bacteria interaction; however, the mechanism behind the association is still unclear. In this study, we used a proteomic approach to investigate the root proteome of Zea mays (cv. DKB240) inoculated with Herbaspirillum seropedicae strain SmR1 grown in vitro and harvested 7 days after inoculation. Eighteen differentially accumulated proteins were observed in root samples, ten of which were identified by MALDI-TOF mass spectrometry peptide mass fingerprint. Among the identified proteins, we observed three proteins present exclusively in inoculated root samples and six upregulated proteins and one downregulated protein relative to control. Differentially expressed maize proteins were identified as hypothetical protein ZEAMMB73_483204, hypothetical protein ZEAMMB73_269466, and tubulin beta-7 chain. The following were identified as H. seropedicae proteins: peroxiredoxin protein, EF-Tu elongation factor protein, cation transport ATPase, NADPH:quinone oxidoreductase, dinitrogenase reductase, and type III secretion ATP synthase. Our results presented the first evidence of type III secretion ATP synthase expression during H. seropedicae-maize root interaction.

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Acknowledgments

The present study was financially supported by the National Institute of Science and Technology—Biological Nitrogen Fixation, INCT FBN, National Council for Scientific and Technological Development, CNPq, and Ministry of Science and Technology, Brazil. CSF is a recipient of PDJ fellowship from CNPq. FPA is a recipient of PhD fellowship from INCT FBN, CAPES, Ministry of Education, Brazil. PAVN is a recipient of PNPD fellowship from CAPES. ACMA is a recipient of research fellowship (PQ-2) from CNPq. We would like to express our gratitude to Dr. Fábio de Oliveira Pedrosa, Universidade Federal do Paraná, for providing H. seropedicae strain.

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  1. Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil

    Cibele Santos Ferrari, Fernanda Plucani Amaral, Jessica Cavalheiro Ferreira Bueno, Mirella Christine Scariot, Pedro Alexandre Valentim-Neto & Ana Carolina Maisonnave Arisi

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Ferrari, C.S., Amaral, F.P., Bueno, J.C.F. et al. Expressed Proteins of Herbaspirillum seropedicae in Maize (DKB240) Roots-Bacteria Interaction Revealed Using Proteomics. Appl Biochem Biotechnol 174, 2267–2277 (2014). https://doi.org/10.1007/s12010-014-1197-3

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