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Nymphaea rubra Ameliorates TNF-α-Induced Insulin Resistance via Suppression of c-Jun NH2-Terminal Kinase and Nuclear Factor-κB in the Rat Skeletal Muscle Cells

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Abstract

In this work, we demonstrated insulin signaling and the anti-inflammatory effects by the chloroform fraction of ethanolic extract of Nymphaea rubra flowers in TNF-α-induced insulin resistance in the rat skeletal muscle cell line (L6 myotubes) to dissect out its anti-hyperglycemic mechanism. N. rubra enhances the GLUT4-mediated glucose transport in a dose dependent manner and also increases the tyrosine phosphorylation of both IR-β and IRS-1, and the IRS-1 associated PI-3 kinase activity in TNF-α-treated L6 myotubes. Moreover, N. rubra decreases Ser307 phosphorylation of IRS-1 by the suppression of JNK and NF-κB activation. In conclusion, N. rubra reverses the insulin resistance by the inhibition of c-Jun NH2-Terminal Kinase and Nuclear-κB.

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Abbreviations

CFEE-Nr:

Chloroform fraction of ethanolic extract of Nymphaea rubra flowers

TNF-α:

Tumor necrosis factor-α

JNK:

c-Jun NH2-terminal kinase

MAPK:

Mitogen-activated protein kinase

NF-κB:

Nuclear factor-κB

IκBα:

Inhibitor of nuclear factor kappa-B

IKKα:

Kinase subunit alpha

IKKβ:

Inhibitor of nuclear factor kappa-B kinase subunit beta

IR-β:

Insulin receptor-β

IRS-1:

Insulin receptor substrate-1

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Acknowledgments

The authors gratefully acknowledge the financial support from the network project of CSIR "Biodiscovery, BSC-0120", New Delhi, India. Two of our authors (SG and NR) gratefully acknowledge to ICMR and CSIR, New Delhi, India, for providing them Senior Research Fellowships. The authors would like to thank the director of CSIR-CDRI for his keen interest in this study. This manuscript bears the CDRI Communication no. 8776.

Conflict of Interest

None.

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Authors and Affiliations

  1. Division of Biochemistry, CSIR-Central Drug Research Institute, Lucknow, 226031, India

    Sudeep Gautam, Neha Rahuja, Nayab Ishrat & Arvind Kumar Srivastava

  2. Medicinal and Process Chemistry, CSIR-Central Drug Research Institute, Lucknow, 226031, India

    R. K. Asthana & Rakesh Maurya

  3. Division of Botany, CSIR-Central Drug Research Institute, Lucknow, 226031, India

    D. K. Mishra

  4. Department of Biotechnology, Jamia Hamdard University, New Delhi, 110062, India

    Swatantra Kumar Jain

Authors
  1. Sudeep Gautam
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  2. Neha Rahuja
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  3. Nayab Ishrat
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  4. R. K. Asthana
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  5. D. K. Mishra
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  6. Rakesh Maurya
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  7. Swatantra Kumar Jain
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  8. Arvind Kumar Srivastava
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Corresponding author

Correspondence to Arvind Kumar Srivastava.

Additional information

Sudeep Gautam and Neha Rahuja contributed equally to this study.

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Gautam, S., Rahuja, N., Ishrat, N. et al. Nymphaea rubra Ameliorates TNF-α-Induced Insulin Resistance via Suppression of c-Jun NH2-Terminal Kinase and Nuclear Factor-κB in the Rat Skeletal Muscle Cells. Appl Biochem Biotechnol 174, 2446–2457 (2014). https://doi.org/10.1007/s12010-014-1192-8

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  • DOI: https://doi.org/10.1007/s12010-014-1192-8

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