Advertisement

Applied Biochemistry and Biotechnology

, Volume 174, Issue 6, pp 2086–2095 | Cite as

Optimization of Adventitious Root Culture for Production of Biomass and Secondary Metabolites in Prunella vulgaris L.

  • Hina Fazal
  • Bilal Haider AbbasiEmail author
  • Nisar Ahmad
Article

Abstract

Adventitious root cultures of Prunella vulgaris L. were established in shaking flask system for the production of biomass and secondary metabolites. Adventitious root cultures were induced from callus cultures obtained from leaf explants on solid Murashige and Skoog (MS) medium containing combination of 6-benzyladenine (BA; 1.0 mg l−1) and naphthalene acetic acid (NAA; 1.5 mg l−1). Thereafter, 0.49 g inoculum was transferred to liquid MS medium supplemented with different concentrations of NAA (0.5–2.0 mg l−1). Growth kinetics of adventitious roots was recorded with an interval of 7 days for 49 days period. Highest biomass accumulation (2.13 g/l) was observed in liquid medium containing 1.0 mg l−1 NAA after 21 days of inoculation. However, other concentrations of NAA also showed similar accumulation pattern but the biomass gradually decreases after 49 days of inoculation. Adventitious roots were collected and dried for investigation of total phenolics (TP), total flavonoids (TF), and antioxidant activities. Higher TPC (0.995 GAE mg/g-DRB) and TFC (6.615 RE mg/g-DRB) were observed in 0.5 mg l−1 NAA treated cultures. In contrast, higher antioxidant activity (83.53 %) was observed 1.5 mg l−1 NAA treated cultures. These results are helpful in up scaling of root cultures into bioreactor for secondary metabolites production.

Keywords

Prunella vulgaris Adventitious root culture Naphthalene acetic acid Biomass Secondary metabolites Antioxidant activity 

Notes

Acknowledgments

We acknowledge the support of Higher Education Commission of Pakistan (HEC) for financial support.

References

  1. 1.
    Ali, M., Abbasi, B. H., & Haq, I. U. (2013). Industrial Crops and Products, 49, 400–406.CrossRefGoogle Scholar
  2. 2.
    Ahmad, N., Abbasi, B. H., Fazal, H., Khan, M. A., & Afridi, M. S. (2014). Comptes Rendus Biologies, 337, 19–28.CrossRefGoogle Scholar
  3. 3.
    Kolewe, M. E., Gaurav, V., & Roberts, S. C. (2008). Molecular Pharmacy, 5, 243–256.CrossRefGoogle Scholar
  4. 4.
    Baque, M. A., Elgirban, A., Lee, E. J., & Paek, K. Y. (2012). Acta Physiology Plantarum, 34, 405–415.CrossRefGoogle Scholar
  5. 5.
    Murthy, H. N., Hahn, E. J., & Paek, K. Y. (2008). Chinese Journal of Biotechnology, 24, 711–716.CrossRefGoogle Scholar
  6. 6.
    Ciésla, Ł., Kowalska, I., Oleszek, W., & Stochmal, A. (2012). Phytochemical Analysis. doi: 10.1002/pca.2379.Google Scholar
  7. 7.
    Ahmad, N., Fazal, H., Abbasi, B. H., & Ali, M. (2013). Forest Systems, 22, 559–563.CrossRefGoogle Scholar
  8. 8.
    Ahmad, N., Abbasi, B. H., Fazal, H., & Rahman, U. R. (2013). Applied Biochemistry and Biotechnology, 169, 2004–2015.CrossRefGoogle Scholar
  9. 9.
    Harborne, J. B. (2001). Natural Product Reports, 18, 361–379.CrossRefGoogle Scholar
  10. 10.
    Matkowski, A. (2006). NATO science series: Life and behavioral sciences (pp. 129–148). Amsterdam: IOS Press.Google Scholar
  11. 11.
    Joo, S. S., Kim, Y., & Lee, D. I. (2010). Plant Pathology Journal, 26, 57–62.CrossRefGoogle Scholar
  12. 12.
    Ahmad, N., Abbasi, B. H., & Fazal, H. (2013). Industrial Crops and Products, 49, 164–168.CrossRefGoogle Scholar
  13. 13.
    Amid, A., Johan, N. N., Jamal, P., & Zain, W. N. W. M. (2011). African Journal of Biotechnology, 10, 18653–18656.Google Scholar
  14. 14.
    Matkowski, A. (2008). Biotechnology Advances, 26, 548–560.CrossRefGoogle Scholar
  15. 15.
    Khan, M. A., Abbasi, B. H., Ahmed, N., & Ali, H. (2013). Industrial Crops and Products, 46, 105–110.CrossRefGoogle Scholar
  16. 16.
    Wang, J., Man, S., Gao, W., Zhang, L., & Huang, L. (2013). Industrial Crops and Products, 41, 57–63.CrossRefGoogle Scholar
  17. 17.
    Baque, M. A., Shiragi, M. H. K., Moh, S. H., Lee, E. J., & Paek, K. Y. (2013). In Vitro Cellular and Developmental Biology-Plant, 49, 737–749.CrossRefGoogle Scholar
  18. 18.
    Cui, X.-H., Chakrabarty, D., Lee, E. J., & Paek, K. Y. (2010). Bioresource Technology, 101, 4708–4716.CrossRefGoogle Scholar
  19. 19.
    Sivanandhan, G., Arun, M., Mayavan, S., Rajesh, M., Mariashibu, T. S., Manickavasagam, M., Selvaraj, N., & Ganapathi, A. (2012). Industrial Crops and Products, 37, 124–129.CrossRefGoogle Scholar
  20. 20.
    Chen, Y., Yu, M., Zhu, Z., Zhang, L., & Guo, Q. (2013). PLoS ONE, 8, 1–7.Google Scholar
  21. 21.
    Rasool, R., Kamili, A. N., Ganai, B. A., & Akbar, S. (2009). Journal of Natural Sciences and Mathematics, Qassim University, 3, 21–26.Google Scholar
  22. 22.
    Chang, R. S., & Yeung, H. W. (1988). Antiviral Research, 9, 163–176.CrossRefGoogle Scholar
  23. 23.
    Tabba, H. D., Chang, R. S., & Smith, K. M. (1989). Antiviral Research, 11, 263–273.CrossRefGoogle Scholar
  24. 24.
    Chen, C. Y., Wu, G., & Zhang, M. Z. (2009). Chinese-German Journal of Clinical Oncology, 8, 426–429.CrossRefGoogle Scholar
  25. 25.
    Chlopcíková, S., Psotová, J., Miketová, P., Sousek, J., Lichnovský, & Miketová, V. (2005). Fitoterapia, 76, 556–561.CrossRefGoogle Scholar
  26. 26.
    Huang, R., Zhao, M., Yang, X., Huang, J., Yang, Y., Chen, B., Tan, J., Huang, J., Li, Z., Lv, Y., & Ji, G. (2013). PLoS ONE, 8, e77355.CrossRefGoogle Scholar
  27. 27.
    Liu, G. M., Jia, X. B., Wang, H. B., Feng, L., & Chen, Y. (2009). Journal of Chinese Medicine Materials, 3, 1920–1926.Google Scholar
  28. 28.
    Zdarilova, A., Svobodova, A., Simanek, V., & Ulrichova, J. (2009). Toxicology In Vitro, 23, 386–392.CrossRefGoogle Scholar
  29. 29.
    Murashige, T., & Skoog, F. (1962). Physiology Plantarum, 15, 473–497.CrossRefGoogle Scholar
  30. 30.
    Lee, Y. S., Yang, T. J., Park, S. U., Baek, J. H., Wu, S. Q., & Lim, K. B. (2011). Plant Omics Journal, 4, 190–194.Google Scholar
  31. 31.
    Taylor, J. L. S., & van-Staden, J. (1998). Plant Growth Regulation, 26, 77–83.CrossRefGoogle Scholar
  32. 32.
    Hussein, S., Ling, A. P. K., Ng, T. H., Ibrahim, R., & Paek, K. Y. (2012). Romanian Biotechnological Letters, 17, 7026–7035.Google Scholar
  33. 33.
    Durkovic, J., & Bukovska, J. (2009). Biologia Plantarum, 53, 715–718.CrossRefGoogle Scholar
  34. 34.
    Thakur, R. C., & Karnosky, D. F. (2007). Plant Cell Reports, 26, 1171–1177.CrossRefGoogle Scholar
  35. 35.
    Peeters, A. J. M., Gerads, W., Barendse, G. W. M., & Wullems, G. J. (1991). Plant Physiology, 97, 402–408.CrossRefGoogle Scholar
  36. 36.
    Van Der Krieken, W. M., Breteler, H., Visser, M. H. M., & Mavridou, D. (1993). Plant Cell Reports, 12, 203–206.CrossRefGoogle Scholar
  37. 37.
    Ali, M., & Abbasi, B. H. (2013). Applied Biochemistry and Biotechnology. doi: 10.1007/s12010-013-0663-7.Google Scholar
  38. 38.
    Palacioa, L., Canterob, J. J., Cusidoc, R. M., & Goleniowski, M. E. (2012). Plant Science, 193–194, 1–7.CrossRefGoogle Scholar
  39. 39.
    Rao, S. R., & Ravishankar, G. A. (2002). Biotechnology Advances, 20, 101–153.CrossRefGoogle Scholar
  40. 40.
    Verpoorte, R., Contin, A., & Memelink, J. (2002). Phytochemistry Reviews, 1, 13–25.CrossRefGoogle Scholar
  41. 41.
    Ribeiro Affonso, V., Ribeiro Bizzo, H., Salgueiro Lage, C. L., & Sato, A. (2009). Journal of Agricultural and Food Chemistry, 57, 6392–6395.CrossRefGoogle Scholar
  42. 42.
    Dornenburg, H., & Knorr, D. (1995). Enzyme Microbiology and Technology, 17, 674–684.CrossRefGoogle Scholar
  43. 43.
    Chan, L. K., Dewi, P. R., & Boey, P. L. (2005). Journal of Plant Biology, 48, 142–145.CrossRefGoogle Scholar
  44. 44.
    Kim, Y. S., Hahn, E. J., Yeung, E. C., & Paek, K. Y. (2003). In Vitro Cellular and Developmental Biology–Plant, 39, 245–249.CrossRefGoogle Scholar
  45. 45.
    Wu, C. H., Dewir, Y. H., Hahn, E. J., & Paek, K. Y. (2006). Journal of Plant Biology, 49, 193–199.CrossRefGoogle Scholar
  46. 46.
    Lee, E. J. (2009). Ph.D. Thesis, Chungbuk National University, Cheong-JuGoogle Scholar
  47. 47.
    Aliyu, A. B., Ibrahim, M. A., Ibrahim, H., Musa, A. M., Lawal, A. Y., Oshanimi, J. A., Usman, M., Abdulkadir, I. E., Oyewale, A. O., & Amupitan, J. O. (2012). Romanian Biotechnological Letters, 17, 7458–7465.Google Scholar
  48. 48.
    Shoji, H., Yamashiro, Y., & Koletzko, B. (2008). In H. S. Packer (Ed.), (p. 72) Boca Raton: CRC Press Taylor & Francis Group.Google Scholar
  49. 49.
    Gupta, V. K., & Sharma, S. K. (2010). International Journal of Biology and Chemistry, 4, 134–140.CrossRefGoogle Scholar
  50. 50.
    Tariq, U., Ali, M., & Abbasi, B. H. (2014). Journal of Photochemistry and Photobiology B: Biology, 5, 264–271.CrossRefGoogle Scholar
  51. 51.
    Lo, S.-F., Nalawade, S. M., Mulabagal, V., Matthew, S., Chen, C.-L., Kuo, C.-L., & Tsay, H.-S. (2004). Biological and Pharmaceutical Bulletin, 27, 731–735.CrossRefGoogle Scholar
  52. 52.
    Güllüce, M., Sökmen, M., Daferera, D., Ağar, G., Özkan, H., Kartal, N., Polissiou, M., Sökmen, A., & Şahin, F. (2003). Journal of Agriculture and Food Chemistry, 51, 3958–3965.CrossRefGoogle Scholar
  53. 53.
    Grzegorczyk, I., Matkowski, A., & Wysokińska, H. (2007). Food Chemistry, 104, 536–541.CrossRefGoogle Scholar
  54. 54.
    Abouzid, S. F., El-Bassuon, A. A., Nasib, A., Khan, S., Qureshi, J., & Choudhary, M. I. (2010). International Journal of Applied Research of Natural Products, 3, 23–27.Google Scholar
  55. 55.
    Duangporn, P., & Siripong, P. (2009). American-Eurasian Journal of Agriculture and Environmental Sciences, 5, 258–263.Google Scholar
  56. 56.
    Seitz, H. U., & Hinderer, W. (1998). 5, 49–76 San Diego: Academic.Google Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Hina Fazal
    • 1
    • 3
  • Bilal Haider Abbasi
    • 2
    Email author
  • Nisar Ahmad
    • 2
  1. 1.Department of Plant Sciences, Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabadPakistan
  2. 2.Department of Biotechnology, Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabadPakistan
  3. 3.Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories ComplexPeshawarPakistan

Personalised recommendations