Applied Biochemistry and Biotechnology

, Volume 174, Issue 6, pp 2058–2066 | Cite as

Surface Modification of Polyacrylonitrile Fibre by Nitrile Hydratase from Corynebacterium nitrilophilus

  • Sheng Chen
  • Huihui Gao
  • Jian Chen
  • Jing WuEmail author


Previously, nitrile hydratase (NHase) from Corynebacterium nitrilophilus was obtained and showed potential in polyacrylonitrile (PAN) fibre modification. In the present study, the modification conditions of C. nitrilophilus NHase on PAN were investigated. In the optimal conditions, the wettability and dyeability (anionic and reactive dyes) of PAN treated by C. nitrilophilus NHase reached a similar level of those treated by alkali. In addition, the chemical composition and microscopically observable were changed in the PAN surface after NHase treatment. Meanwhile, it revealed that cutinase combined with NHase facilitates the PAN hydrolysis slightly because of the ester existed in PAN as co-monomer was hydrolyzed. All these results demonstrated that C. nitrilophilus NHase can modify PAN efficiently without textile structure damage, and this study provides a foundation for the further application of C. nitrilophilus NHase in PAN modification industry.


Corynebacterium nitrilophilus Nitrile hydratase Polyacrylonitrile Modification 



This work was supported financially by the Science and Technology Support Project of Jiangsu Province (BE2012018 and BE2012019).


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Sheng Chen
    • 1
    • 2
  • Huihui Gao
    • 1
    • 2
  • Jian Chen
    • 1
    • 2
  • Jing Wu
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiChina
  2. 2.School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan UniversityWuxiChina

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